DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE
Forensic chemical examination is carried out in order to isolate, identify and quantify toxic, narcotic, psychotropic and potent substances in the organs and fluids of the human body. The present study we researched the process of extraction of 2,3-benzodiazepine - tofisopam. There have been cases o...
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Scientific Сentre for Family Health and Human Reproduction Problems
2017-02-01
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doaj-d7012c13891d44c49079b742a1b0f7972021-08-17T13:52:56ZrusScientific Сentre for Family Health and Human Reproduction ProblemsActa Biomedica Scientifica2541-94202587-95962017-02-0121525510.12737/article_5955e6b4db2812.30407534362DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTUREA. M. Lazitskaya0N. V. Chmelevskaya1E. A. Illarionova2Irkutsk Regional Agency of Forensic Medical ExaminationIrkutsk Regional Agency of Forensic Medical ExaminationIrkutsk State Medical UniversityForensic chemical examination is carried out in order to isolate, identify and quantify toxic, narcotic, psychotropic and potent substances in the organs and fluids of the human body. The present study we researched the process of extraction of 2,3-benzodiazepine - tofisopam. There have been cases of tofisopam poisoning. And there are no descriptions of tofisopam isolation from biological fluids in the literature. Liquid extraction, an effective and commonly used method of isolation in the chemical-toxicological analysis, is used when extracting substances from blood, lymph, saliva, urine and stomach washings. The process of isolation may be influenced by the nature of the organic solvent, pH, the presence of an electrolyte, time and frequency of extraction. We have studied the effect of each factor on tofisopam isolation from aqueous solutions. It has been found that the optimal organic solvent for the extraction of the solutions is dichloroethane, which extracts maximum amount of the analyte at pH = 5.0. Saturated sodium sulphate has a salting-in effect for tofisopam. The optimum conditions for tofisopam isolating are 7 minutes with double extraction. Based on the research method of tofisopam isolating the model mixture of urine was developed. We found that this model urine mixture isolates 66.51-67.9l % of tofisopam. We performed validation assessment of the developed method for evaluation of precision and accuracy. The assessment showed the suitability of techniques for the analysis.https://www.actabiomedica.ru/jour/article/view/362chemical and toxicological analysisisolationtofisopam |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
A. M. Lazitskaya N. V. Chmelevskaya E. A. Illarionova |
spellingShingle |
A. M. Lazitskaya N. V. Chmelevskaya E. A. Illarionova DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE Acta Biomedica Scientifica chemical and toxicological analysis isolation tofisopam |
author_facet |
A. M. Lazitskaya N. V. Chmelevskaya E. A. Illarionova |
author_sort |
A. M. Lazitskaya |
title |
DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE |
title_short |
DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE |
title_full |
DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE |
title_fullStr |
DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE |
title_full_unstemmed |
DEVELOPMENT OF METHODS OF TOFISOPAM ISOLATION IN MODEL URINE MIXTURE |
title_sort |
development of methods of tofisopam isolation in model urine mixture |
publisher |
Scientific Сentre for Family Health and Human Reproduction Problems |
series |
Acta Biomedica Scientifica |
issn |
2541-9420 2587-9596 |
publishDate |
2017-02-01 |
description |
Forensic chemical examination is carried out in order to isolate, identify and quantify toxic, narcotic, psychotropic and potent substances in the organs and fluids of the human body. The present study we researched the process of extraction of 2,3-benzodiazepine - tofisopam. There have been cases of tofisopam poisoning. And there are no descriptions of tofisopam isolation from biological fluids in the literature. Liquid extraction, an effective and commonly used method of isolation in the chemical-toxicological analysis, is used when extracting substances from blood, lymph, saliva, urine and stomach washings. The process of isolation may be influenced by the nature of the organic solvent, pH, the presence of an electrolyte, time and frequency of extraction. We have studied the effect of each factor on tofisopam isolation from aqueous solutions. It has been found that the optimal organic solvent for the extraction of the solutions is dichloroethane, which extracts maximum amount of the analyte at pH = 5.0. Saturated sodium sulphate has a salting-in effect for tofisopam. The optimum conditions for tofisopam isolating are 7 minutes with double extraction. Based on the research method of tofisopam isolating the model mixture of urine was developed. We found that this model urine mixture isolates 66.51-67.9l % of tofisopam. We performed validation assessment of the developed method for evaluation of precision and accuracy. The assessment showed the suitability of techniques for the analysis. |
topic |
chemical and toxicological analysis isolation tofisopam |
url |
https://www.actabiomedica.ru/jour/article/view/362 |
work_keys_str_mv |
AT amlazitskaya developmentofmethodsoftofisopamisolationinmodelurinemixture AT nvchmelevskaya developmentofmethodsoftofisopamisolationinmodelurinemixture AT eaillarionova developmentofmethodsoftofisopamisolationinmodelurinemixture |
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1721204587758616576 |