Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats
Abstract Chronic lymphocytic thyroiditis (CLT) is a common autoimmune disorder. The possible pathogenic role and mechanism of dibutyl phthalate (DBP) in CLT is still controversial. Experiments were conducted after 35-days of oral exposure to the three concentrations of DBP or saline, and three immun...
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doaj-d716502762ea4ed0b6d02367c98ce3302020-12-08T01:40:08ZengNature Publishing GroupScientific Reports2045-23222017-11-017111010.1038/s41598-017-15533-zOral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar ratsYang Wu0Jinquan Li1Biao Yan2Yuqing Zhu3Xudong Liu4Mingqing Chen5Dai Li6Ching-Chang Lee7Xu Yang8Ping Ma9Laboratory of Environment- immunological and neurological Diseases, School of Basic Medical Sciences, Hubei University of Science and TechnologyLaboratory of Environmental Biomedicine, College of Life Sciences, Central China Normal UniversityLaboratory of Environment- immunological and neurological Diseases, School of Basic Medical Sciences, Hubei University of Science and TechnologyLaboratory of Environmental Biomedicine, College of Life Sciences, Central China Normal UniversityLaboratory of Environmental Biomedicine, College of Life Sciences, Central China Normal UniversityLaboratory of Environmental Biomedicine, College of Life Sciences, Central China Normal UniversityLaboratory of Environment- immunological and neurological Diseases, School of Basic Medical Sciences, Hubei University of Science and TechnologyDepartment of Environmental and Occupational Health, Medical College, National Cheng Kung UniversityLaboratory of Environment- immunological and neurological Diseases, School of Basic Medical Sciences, Hubei University of Science and TechnologyLaboratory of Environment- immunological and neurological Diseases, School of Basic Medical Sciences, Hubei University of Science and TechnologyAbstract Chronic lymphocytic thyroiditis (CLT) is a common autoimmune disorder. The possible pathogenic role and mechanism of dibutyl phthalate (DBP) in CLT is still controversial. Experiments were conducted after 35-days of oral exposure to the three concentrations of DBP or saline, and three immunizations with thyroglobulin (TG). Healthy female Wistar rats were randomly divided into ten exposure groups (n = 8 each): (A) saline control, (B) 0.5 mg/kg/d DBP, (C) 5 mg/kg/d DBP, (D) 50 mg/kg/d DBP, (E) TG-immunized group, (F) TG- combined with 0.5 mg/kg/d DBP, (G) TG- combined with 5 mg/kg/d DBP, (H) TG- combined with 50 mg/kg/d DBP, (I) TG- combined with 50 mg/kg/d DBP plus 100 mg/kg/d vitamin C; (J) 100 mg/kg/d vitamin C. We showed that oral exposure DBP can aggravate CLT in rats. This deterioration was concomitant with increased thyroid auto antibodies, Th1/Th2 imbalance and Th17 immune response, activated pro-inflammatory and apoptosis pathways, and increased thyroid dysfunction in rats. Our results also suggested that DBP could promote oxidative damage. The study also found that vitamin C reduced the levels of oxidative stress and alleviated CLT. In short, the study showed that DBP exacerbated CLT through oxidative stress.https://doi.org/10.1038/s41598-017-15533-z |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yang Wu Jinquan Li Biao Yan Yuqing Zhu Xudong Liu Mingqing Chen Dai Li Ching-Chang Lee Xu Yang Ping Ma |
spellingShingle |
Yang Wu Jinquan Li Biao Yan Yuqing Zhu Xudong Liu Mingqing Chen Dai Li Ching-Chang Lee Xu Yang Ping Ma Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats Scientific Reports |
author_facet |
Yang Wu Jinquan Li Biao Yan Yuqing Zhu Xudong Liu Mingqing Chen Dai Li Ching-Chang Lee Xu Yang Ping Ma |
author_sort |
Yang Wu |
title |
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats |
title_short |
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats |
title_full |
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats |
title_fullStr |
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats |
title_full_unstemmed |
Oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female Wistar rats |
title_sort |
oral exposure to dibutyl phthalate exacerbates chronic lymphocytic thyroiditis through oxidative stress in female wistar rats |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2017-11-01 |
description |
Abstract Chronic lymphocytic thyroiditis (CLT) is a common autoimmune disorder. The possible pathogenic role and mechanism of dibutyl phthalate (DBP) in CLT is still controversial. Experiments were conducted after 35-days of oral exposure to the three concentrations of DBP or saline, and three immunizations with thyroglobulin (TG). Healthy female Wistar rats were randomly divided into ten exposure groups (n = 8 each): (A) saline control, (B) 0.5 mg/kg/d DBP, (C) 5 mg/kg/d DBP, (D) 50 mg/kg/d DBP, (E) TG-immunized group, (F) TG- combined with 0.5 mg/kg/d DBP, (G) TG- combined with 5 mg/kg/d DBP, (H) TG- combined with 50 mg/kg/d DBP, (I) TG- combined with 50 mg/kg/d DBP plus 100 mg/kg/d vitamin C; (J) 100 mg/kg/d vitamin C. We showed that oral exposure DBP can aggravate CLT in rats. This deterioration was concomitant with increased thyroid auto antibodies, Th1/Th2 imbalance and Th17 immune response, activated pro-inflammatory and apoptosis pathways, and increased thyroid dysfunction in rats. Our results also suggested that DBP could promote oxidative damage. The study also found that vitamin C reduced the levels of oxidative stress and alleviated CLT. In short, the study showed that DBP exacerbated CLT through oxidative stress. |
url |
https://doi.org/10.1038/s41598-017-15533-z |
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