FUNGAL DIVERSITY IN LIMA BEAN SEEDS / DIVERSIDADE FÚNGICA EM SEMENTES DE FEIJÃO-FAVA

• Main Authors: J. M. Mota, M. P. Melo, F. F. S. Silva, E. M. J. Sousa, E. S. Sousa, B. M. Barguil, J. E. A. Beserra Jr
• Format: Article
• Language: English
• Published: Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) 2017-03-01
• Series: Revista Brasileira de Engenharia de Biossistemas
• Online Access: http://seer.tupa.unesp.br/index.php/BIOENG/article/view/490
• ISSN: 1981-7061; 2359-6724

<p>There is no comprehensive survey of the presence of fungi associated with lima bean seeds. The objective of this study was to evaluate the fungal diversity of 34 samples of lima bean seeds, acquired with farmers and markets during the years 2014 and 2015 in the states of Ceará, Maranhão, Paraíba and Piauí. Subsamples of 400 seeds were sterilized by soaking in 1% NaOCl solution for 3 minutes, followed by two washes in sterile distilled water, and dried at room temperature. Seeds were placed in Petri plates containing Potato Dextrose Agar, and incubated at 25 °C for seven days. Fungal identification was based on morphological markers, and its incidences were quantified. Isolates from the main phytopathogens were also identified by the amplification and sequencing of housekeeping genes. Samples presented variations in diversity and incidence, with the presence of 22 fungal genera. <em>Aspergillus</em> spp., <em>Penicillium</em> spp., <em>Curvularia</em> sp. and <em>Monilinia</em> sp. corresponded to 63.76% of the colonies observed. Among the phytopathogens, isolates of <em>Macrophomina phaseolina</em>, <em>Colletotrichum truncatum</em>, <em>Rhizoctonia solani</em>, <em>Fusarium udum</em> and <em>Fusarium oxysporum</em> were identified by BLAST<em>n</em> analysis (99 to 100% DNA similarity) and phylogenetic analysis. <em>C. truncatum</em> and <em>M. phaseolina</em>presented the highest incidences (0.95% and 1.58%, respectively) among phytopathogens.</p><div><div><p> </p></div></div>