Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis

Introduction: Dental caries (tooth decay) remains one of the most common chronic infectious disease in the world. Disclosure of camouflaged cariogenic bacteria will be a great motivation for better oral hygiene. The Hypothesis: At present, lux transposon cassette, Tn4001 luxABCDE Kmr, is available t...

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Main Author: Jafar Kolahi
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2016-01-01
Series:Dental Hypotheses
Subjects:
Online Access:http://www.dentalhypotheses.com/article.asp?issn=2155-8213;year=2016;volume=7;issue=1;spage=12;epage=14;aulast=Kolahi
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spelling doaj-d7256717f7c64ed5a35a1b659ae65b4d2020-11-24T22:30:45ZengWolters Kluwer Medknow PublicationsDental Hypotheses2155-82132016-01-0171121410.4103/2155-8213.177404Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesisJafar KolahiIntroduction: Dental caries (tooth decay) remains one of the most common chronic infectious disease in the world. Disclosure of camouflaged cariogenic bacteria will be a great motivation for better oral hygiene. The Hypothesis: At present, lux transposon cassette, Tn4001 luxABCDE Kmr, is available that could be used for stable bioluminescent transformation of a wide range of gram-positive bacteria, e.g. Streptococcus mutans and Lactobacillus. After this step, sensitive charge-coupled device (CCD) camera could be used to detect the low levels of light emitted from bioluminescent cariogenic bacteria. Living imaging software would be used for analysis and three-dimensional (3D) reconstruction of images. Evaluation of the Hypothesis: Entrance of transgenic organisms into the oral cavity should be done with great caution. Ethical consideration is necessary and primary animal studies are required. The main limitation of this technique will be oxygen. As mentioned previously, bioluminescent reactions need oxygen. Hence, bioluminescent imaging cannot be used for anaerobic bacteria, e.g., Streptococcus sobrinus.http://www.dentalhypotheses.com/article.asp?issn=2155-8213;year=2016;volume=7;issue=1;spage=12;epage=14;aulast=KolahiBiodonticsbioluminescent imagingdental cariesStreptococcus mutansLactobacillus
collection DOAJ
language English
format Article
sources DOAJ
author Jafar Kolahi
spellingShingle Jafar Kolahi
Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis
Dental Hypotheses
Biodontics
bioluminescent imaging
dental caries
Streptococcus mutans
Lactobacillus
author_facet Jafar Kolahi
author_sort Jafar Kolahi
title Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis
title_short Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis
title_full Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis
title_fullStr Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis
title_full_unstemmed Real-time monitoring of cariogenic bacteria via bioluminescent imaging: A biodontic hypothesis
title_sort real-time monitoring of cariogenic bacteria via bioluminescent imaging: a biodontic hypothesis
publisher Wolters Kluwer Medknow Publications
series Dental Hypotheses
issn 2155-8213
publishDate 2016-01-01
description Introduction: Dental caries (tooth decay) remains one of the most common chronic infectious disease in the world. Disclosure of camouflaged cariogenic bacteria will be a great motivation for better oral hygiene. The Hypothesis: At present, lux transposon cassette, Tn4001 luxABCDE Kmr, is available that could be used for stable bioluminescent transformation of a wide range of gram-positive bacteria, e.g. Streptococcus mutans and Lactobacillus. After this step, sensitive charge-coupled device (CCD) camera could be used to detect the low levels of light emitted from bioluminescent cariogenic bacteria. Living imaging software would be used for analysis and three-dimensional (3D) reconstruction of images. Evaluation of the Hypothesis: Entrance of transgenic organisms into the oral cavity should be done with great caution. Ethical consideration is necessary and primary animal studies are required. The main limitation of this technique will be oxygen. As mentioned previously, bioluminescent reactions need oxygen. Hence, bioluminescent imaging cannot be used for anaerobic bacteria, e.g., Streptococcus sobrinus.
topic Biodontics
bioluminescent imaging
dental caries
Streptococcus mutans
Lactobacillus
url http://www.dentalhypotheses.com/article.asp?issn=2155-8213;year=2016;volume=7;issue=1;spage=12;epage=14;aulast=Kolahi
work_keys_str_mv AT jafarkolahi realtimemonitoringofcariogenicbacteriaviabioluminescentimagingabiodontichypothesis
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