The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model
AIM: To investigate the therapeutic efficiency of Mulberry eye drops on dry eye in perimenopausal female rabbit model. <p>METHODS: Totally 36 female New Zealand white rabbits were selected and operated with bilateral ovariectomy and fed for 60d postoperatively to make perimenopausal female rab...
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Press of International Journal of Ophthalmology (IJO PRESS)
2020-03-01
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Online Access: | http://ies.ijo.cn/cn_publish/2020/3/202003007.pdf |
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Article |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Wei Jiang Qian-Min Ge Biao Li Qing Yuan Rong-Bin Liang Qiu-Yu Li Pei-Wen Zhu Jun-Ping Deng Wen-Qin Shi Yi Shao |
spellingShingle |
Wei Jiang Qian-Min Ge Biao Li Qing Yuan Rong-Bin Liang Qiu-Yu Li Pei-Wen Zhu Jun-Ping Deng Wen-Qin Shi Yi Shao The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model Guoji Yanke Zazhi dry eye mulberry eye drops treatment androgen |
author_facet |
Wei Jiang Qian-Min Ge Biao Li Qing Yuan Rong-Bin Liang Qiu-Yu Li Pei-Wen Zhu Jun-Ping Deng Wen-Qin Shi Yi Shao |
author_sort |
Wei Jiang |
title |
The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model |
title_short |
The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model |
title_full |
The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model |
title_fullStr |
The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model |
title_full_unstemmed |
The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit model |
title_sort |
application of mulberry eye drops on xerophthalmia in perimenopausal female rabbit model |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
Guoji Yanke Zazhi |
issn |
1672-5123 1672-5123 |
publishDate |
2020-03-01 |
description |
AIM: To investigate the therapeutic efficiency of Mulberry eye drops on dry eye in perimenopausal female rabbit model. <p>METHODS: Totally 36 female New Zealand white rabbits were selected and operated with bilateral ovariectomy and fed for 60d postoperatively to make perimenopausal female rabbit dry eye model(36 eyes, all right eyes). And 24 of them were randomly divided into control group and experimental group(12 for each): the control group used PBS, the experimental group used Mulberry eye drops, the other 12 did not use any eye drops. The Schirmer I tests(SⅠt)and corneal fluorescein(FL)were made, and the tear total protein content, amylase activity, lactoferrin and lysozyme contents and confocal scanning microscopy were performed in two groups before treatment and 2, 4, 6wk after treatment. <p>RESULTS: There was no significant difference in SⅠt,FL scores,total proteins,lysozyme,lactoferrin contents and amylase activity between two groups at pre-therapy(all <i>P</i>>0.05).After 2, 4 and 6wk of treatment, there were significant changes in SⅠt and FL scores in the two groups(<i>P</i><0.05). There were no significant changes in lysozyme content, lactoferrin, amylase activity and total tear protein in the experimental group compared with those before treatment(all <i>P</i>>0.05), while there were significant changes in lysozyme content, lactoferrin, amylase activity and total tear protein in the control group(all <i>P</i><0.05). At 2, 4 and 6wk after treatment, there were differences in the scores of SⅠt and FL, lysozyme content, lactoferrin, amylase activity and total protein content in tear between the two groups(<i>P</i><0.05). At 6 wk after treatment, the mean number of corneal basal cells and inflammatory cells in control group were 4436±289mm2 and 321±91mm2,respectively,which in experimental group were 3219±223mm2 and 36±11mm2, respectively,there were statistical differences between two groups( all <i>P</i><0.05). After 6wk treatment, there were no change of corneal nerve bending and less density in control group while the nerve fiber bending and density decreased significantly in experimental group,there were statistical differences between two groups(all <i>P</i><0.05).<p>CONCLUSION: Mulberry eye drops has significantly therapy effects on the dry eye induced by decreased androgen and it has a definite clinical application value. |
topic |
dry eye mulberry eye drops treatment androgen |
url |
http://ies.ijo.cn/cn_publish/2020/3/202003007.pdf |
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doaj-d734606f1d9841ef85e90dfbbdcf2dbb2020-11-25T02:20:21ZengPress of International Journal of Ophthalmology (IJO PRESS)Guoji Yanke Zazhi1672-51231672-51232020-03-0120343243710.3980/j.issn.1672-5123.2020.3.07The application of Mulberry eye drops on xerophthalmia in perimenopausal female rabbit modelWei Jiang0Qian-Min Ge1Biao Li2Qing Yuan3Rong-Bin Liang4Qiu-Yu Li5Pei-Wen Zhu6Jun-Ping Deng7Wen-Qin Shi8Yi Shao9Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China; Department of Ophthalmology, the Gao'an People's Hospital, Gao'an 330800, Jiangxi Province, ChinaCo-first authors: Wei Jiang and Qian-Min Ge.Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the Gao'an People's Hospital, Gao'an 330800, Jiangxi Province, ChinaCo-first authors: Wei Jiang and Qian-Min Ge.Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaDepartment of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, ChinaAIM: To investigate the therapeutic efficiency of Mulberry eye drops on dry eye in perimenopausal female rabbit model. <p>METHODS: Totally 36 female New Zealand white rabbits were selected and operated with bilateral ovariectomy and fed for 60d postoperatively to make perimenopausal female rabbit dry eye model(36 eyes, all right eyes). And 24 of them were randomly divided into control group and experimental group(12 for each): the control group used PBS, the experimental group used Mulberry eye drops, the other 12 did not use any eye drops. The Schirmer I tests(SⅠt)and corneal fluorescein(FL)were made, and the tear total protein content, amylase activity, lactoferrin and lysozyme contents and confocal scanning microscopy were performed in two groups before treatment and 2, 4, 6wk after treatment. <p>RESULTS: There was no significant difference in SⅠt,FL scores,total proteins,lysozyme,lactoferrin contents and amylase activity between two groups at pre-therapy(all <i>P</i>>0.05).After 2, 4 and 6wk of treatment, there were significant changes in SⅠt and FL scores in the two groups(<i>P</i><0.05). There were no significant changes in lysozyme content, lactoferrin, amylase activity and total tear protein in the experimental group compared with those before treatment(all <i>P</i>>0.05), while there were significant changes in lysozyme content, lactoferrin, amylase activity and total tear protein in the control group(all <i>P</i><0.05). At 2, 4 and 6wk after treatment, there were differences in the scores of SⅠt and FL, lysozyme content, lactoferrin, amylase activity and total protein content in tear between the two groups(<i>P</i><0.05). At 6 wk after treatment, the mean number of corneal basal cells and inflammatory cells in control group were 4436±289mm2 and 321±91mm2,respectively,which in experimental group were 3219±223mm2 and 36±11mm2, respectively,there were statistical differences between two groups( all <i>P</i><0.05). After 6wk treatment, there were no change of corneal nerve bending and less density in control group while the nerve fiber bending and density decreased significantly in experimental group,there were statistical differences between two groups(all <i>P</i><0.05).<p>CONCLUSION: Mulberry eye drops has significantly therapy effects on the dry eye induced by decreased androgen and it has a definite clinical application value.http://ies.ijo.cn/cn_publish/2020/3/202003007.pdfdry eyemulberry eye dropstreatmentandrogen |