Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae
Abstract In this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. With the exogenous addition of 0.3% (w/v) SLs in the initial medium, the RML activity reached 430.0 U/mL, an increase of 25.0% compared to th...
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doaj-d749913dbf4449218a7ab3314670b5d82021-09-05T11:14:21ZengSpringerOpenBioresources and Bioprocessing2197-43652021-09-018111010.1186/s40643-021-00433-yEffect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzaeQianqian Zhang0Zhiyue Xiong1Lei Sun2Xiwei Tian3Guiwei Tian4Yiming Yang5Xu Li6Yonghong Wang7Ju Chu8State Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyWilmar Biotechnology R&D Center Co., LtdWilmar Biotechnology R&D Center Co., LtdState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyState Key Laboratory of Bioreactor Engineering, East China University of Science and TechnologyAbstract In this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. With the exogenous addition of 0.3% (w/v) SLs in the initial medium, the RML activity reached 430.0 U/mL, an increase of 25.0% compared to the control group. Subsequently, the physiological metabolic responses of A. oryzae to the addition of SLs were further explored. The results showed that though SLs had almost no effect on the RML secretion, it would affect the morphology of the cells. During the late phase of the fermentation, the proportion of middle pellets, which was generally considered as an energetic and stable state for enzyme production was increased with the addition of SLs. Simultaneously, the viscosity of fermentation broth was reduced, which facilitated the increase of oxygen transfer, thereby improving the RML production. Finally, it could be found that the addition of SLs significantly increased the contents of precursor amino acids, especially for those rank first and second of the RML composition, and it could promote the synthesis of RML.https://doi.org/10.1186/s40643-021-00433-yRhizomucor miehei lipaseAspergillus oryzaeSophorolipidsMorphologyAmino acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Qianqian Zhang Zhiyue Xiong Lei Sun Xiwei Tian Guiwei Tian Yiming Yang Xu Li Yonghong Wang Ju Chu |
spellingShingle |
Qianqian Zhang Zhiyue Xiong Lei Sun Xiwei Tian Guiwei Tian Yiming Yang Xu Li Yonghong Wang Ju Chu Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae Bioresources and Bioprocessing Rhizomucor miehei lipase Aspergillus oryzae Sophorolipids Morphology Amino acid |
author_facet |
Qianqian Zhang Zhiyue Xiong Lei Sun Xiwei Tian Guiwei Tian Yiming Yang Xu Li Yonghong Wang Ju Chu |
author_sort |
Qianqian Zhang |
title |
Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae |
title_short |
Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae |
title_full |
Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae |
title_fullStr |
Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae |
title_full_unstemmed |
Effect of biosurfactant sophorolipids on Rhizomucor miehei lipase fermentation by Aspergillus oryzae |
title_sort |
effect of biosurfactant sophorolipids on rhizomucor miehei lipase fermentation by aspergillus oryzae |
publisher |
SpringerOpen |
series |
Bioresources and Bioprocessing |
issn |
2197-4365 |
publishDate |
2021-09-01 |
description |
Abstract In this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. With the exogenous addition of 0.3% (w/v) SLs in the initial medium, the RML activity reached 430.0 U/mL, an increase of 25.0% compared to the control group. Subsequently, the physiological metabolic responses of A. oryzae to the addition of SLs were further explored. The results showed that though SLs had almost no effect on the RML secretion, it would affect the morphology of the cells. During the late phase of the fermentation, the proportion of middle pellets, which was generally considered as an energetic and stable state for enzyme production was increased with the addition of SLs. Simultaneously, the viscosity of fermentation broth was reduced, which facilitated the increase of oxygen transfer, thereby improving the RML production. Finally, it could be found that the addition of SLs significantly increased the contents of precursor amino acids, especially for those rank first and second of the RML composition, and it could promote the synthesis of RML. |
topic |
Rhizomucor miehei lipase Aspergillus oryzae Sophorolipids Morphology Amino acid |
url |
https://doi.org/10.1186/s40643-021-00433-y |
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