Scanning single-molecule counting system for Eprobe with highly simple and effective approach.
Here, we report a rapid and ultra-sensitive detection technique for fluorescent molecules called scanning single molecular counting (SSMC). The method uses a fluorescence-based digital measurement system to count single molecules in a solution. In this technique, noise is reduced by conforming the s...
Main Authors: | Takeshi Hanami, Tetsuya Tanabe, Takuya Hanashi, Mitsushiro Yamaguchi, Hidetaka Nakata, Yasumasa Mitani, Yasumasa Kimura, Takahiro Soma, Kengo Usui, Michiko Isobe, Takashi Ogawa, Masayoshi Itoh, Yoshihide Hayashizaki, Seiji Kondo |
---|---|
Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020-01-01
|
Series: | PLoS ONE |
Online Access: | https://doi.org/10.1371/journal.pone.0243319 |
Similar Items
-
Eprobe mediated real-time PCR monitoring and melting curve analysis.
by: Takeshi Hanami, et al.
Published: (2013-01-01) -
Eprobe mediated RT-qPCR for the detection of leukemia-associated fusion genes.
by: Koji Tsuchiya, et al.
Published: (2018-01-01) -
Highly sensitive detection of a HER2 12-base pair duplicated insertion mutation in lung cancer using the Eprobe-PCR method.
by: Yoshiaki Takase, et al.
Published: (2017-01-01) -
Edesign: Primer and Enhanced Internal Probe Design Tool for Quantitative PCR Experiments and Genotyping Assays.
by: Yasumasa Kimura, et al.
Published: (2016-01-01) -
Rapid detection of SNP (c.309T>G) in the MDM2 gene by the Duplex SmartAmp method.
by: Yasuaki Enokida, et al.
Published: (2013-01-01)