<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene
<p>Abstract</p> <p>Background</p> <p>Rough lemon (<it>Citrus jambhiri</it> Lush.) is the most commonly used <it>Citrus</it> rootstock in south Asia. It is extremely sensitive to salt stress that decreases the growth and yield of <it>Citrus&...
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doaj-d760d75b3f9e46998040f4f60e006ca42020-11-25T02:54:18ZengBMCBMC Research Notes1756-05002012-06-015128510.1186/1756-0500-5-285<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> geneAli ShawkatMannan AbdulEl Oirdi MohamedWaheed AbdulMirza Bushra<p>Abstract</p> <p>Background</p> <p>Rough lemon (<it>Citrus jambhiri</it> Lush.) is the most commonly used <it>Citrus</it> rootstock in south Asia. It is extremely sensitive to salt stress that decreases the growth and yield of <it>Citrus</it> crops in many areas worldwide. Over expression of the yeast halotolerant gene (<it>HAL2)</it> results in increasing the level of salt tolerance in transgenic plants.</p> <p>Results</p> <p>Transformation of rough lemon was carried out by using <it>Agrobacterium tumefaciens</it> strains LBA4404 harboring plasmid pJRM17. Transgenic shoots were selected on kanamycin 100 mg L<sup>-1</sup> along with 250 mg L<sup>-1</sup> each of cefotaxime and vancomycin for effective inhibition of <it>Agrobacterium</it> growth. The Murashige and Skoog (MS) medium containing 200 μM acetoseryngone (AS) proved to be the best inoculation and co-cultivation medium for transformation. MS medium supplemented with 3 mg L<sup>-1</sup> of 6-benzylaminopurine (BA) showed maximum regeneration efficiency of the transformed explants. The final selection of the transformed plants was made on the basis of PCR and Southern blot analysis.</p> <p>Conclusion</p> <p>Rough lemon has been successfully transformed via <it>Agrobacterium tumefaciens</it> with β-glucuronidase (<it>GUS)</it> and <it>HAL2.</it> Various factors affecting gene transformation and regeneration efficiency were also investigated.</p> http://www.biomedcentral.com/1756-0500/5/1/285Genetic transformationYeast halotolerant gene (<it>HAL2</it>)<it>Citrus jambhiri</it> Lush and <it>Agrobacterium tumefaciens</it> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali Shawkat Mannan Abdul El Oirdi Mohamed Waheed Abdul Mirza Bushra |
spellingShingle |
Ali Shawkat Mannan Abdul El Oirdi Mohamed Waheed Abdul Mirza Bushra <it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene BMC Research Notes Genetic transformation Yeast halotolerant gene (<it>HAL2</it>) <it>Citrus jambhiri</it> Lush and <it>Agrobacterium tumefaciens</it> |
author_facet |
Ali Shawkat Mannan Abdul El Oirdi Mohamed Waheed Abdul Mirza Bushra |
author_sort |
Ali Shawkat |
title |
<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene |
title_short |
<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene |
title_full |
<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene |
title_fullStr |
<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene |
title_full_unstemmed |
<it>Agrobacterium</it>-mediated transformation of rough lemon (<it>Citrus jambhiri</it> Lush) with yeast <it>HAL2</it> gene |
title_sort |
<it>agrobacterium</it>-mediated transformation of rough lemon (<it>citrus jambhiri</it> lush) with yeast <it>hal2</it> gene |
publisher |
BMC |
series |
BMC Research Notes |
issn |
1756-0500 |
publishDate |
2012-06-01 |
description |
<p>Abstract</p> <p>Background</p> <p>Rough lemon (<it>Citrus jambhiri</it> Lush.) is the most commonly used <it>Citrus</it> rootstock in south Asia. It is extremely sensitive to salt stress that decreases the growth and yield of <it>Citrus</it> crops in many areas worldwide. Over expression of the yeast halotolerant gene (<it>HAL2)</it> results in increasing the level of salt tolerance in transgenic plants.</p> <p>Results</p> <p>Transformation of rough lemon was carried out by using <it>Agrobacterium tumefaciens</it> strains LBA4404 harboring plasmid pJRM17. Transgenic shoots were selected on kanamycin 100 mg L<sup>-1</sup> along with 250 mg L<sup>-1</sup> each of cefotaxime and vancomycin for effective inhibition of <it>Agrobacterium</it> growth. The Murashige and Skoog (MS) medium containing 200 μM acetoseryngone (AS) proved to be the best inoculation and co-cultivation medium for transformation. MS medium supplemented with 3 mg L<sup>-1</sup> of 6-benzylaminopurine (BA) showed maximum regeneration efficiency of the transformed explants. The final selection of the transformed plants was made on the basis of PCR and Southern blot analysis.</p> <p>Conclusion</p> <p>Rough lemon has been successfully transformed via <it>Agrobacterium tumefaciens</it> with β-glucuronidase (<it>GUS)</it> and <it>HAL2.</it> Various factors affecting gene transformation and regeneration efficiency were also investigated.</p> |
topic |
Genetic transformation Yeast halotolerant gene (<it>HAL2</it>) <it>Citrus jambhiri</it> Lush and <it>Agrobacterium tumefaciens</it> |
url |
http://www.biomedcentral.com/1756-0500/5/1/285 |
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