Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies
Melatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the exp...
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doaj-d7747e83c548416fbcd7324db1b2f9e32020-11-25T04:01:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01218134813410.3390/ijms21218134Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related PathologiesAnnalucia Carbone0Natalia Linkova1Victoria Polyakova2Ekaterina Mironova3Ulduz Hashimova4Ahmed Gadzhiev5Khatira Safikhanova6Tatiana Kvetnaia Julia Krylova7Roberto Tarquini8Gianluigi Mazzoccoli9Igor Kvetnoy10Department of Medical Sciences, Division of Internal Medicine and Chronobiology Laboratory, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, ItalyDepartment of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, 197110 Saint Petersburg, RussiaLaboratory of Cell Biology and Pathology, Saint Petersburg State Pediatric Medical University, 194100 Saint Petersburg, RussiaDepartment of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, 197110 Saint Petersburg, RussiaGarayev Institute of Physiology, Azerbaijan National Academy of Sciences, Baku AZ1100, AzerbaijanGarayev Institute of Physiology, Azerbaijan National Academy of Sciences, Baku AZ1100, AzerbaijanGarayev Institute of Physiology, Azerbaijan National Academy of Sciences, Baku AZ1100, AzerbaijanDepartment of Biogerontology, Saint Petersburg Institute of Bioregulation and Gerontology, 197110 Saint Petersburg, RussiaDepartment of Clinical and Experimental Medicine, University of Florence, 50134 Florence, ItalyDepartment of Medical Sciences, Division of Internal Medicine and Chronobiology Laboratory, Fondazione IRCCS Casa Sollievo della Sofferenza, 71013 San Giovanni Rotondo, ItalyDepartment of Physiology and Department of Pathology, Saint Petersburg State University, 199034 Saint Petersburg, RussiaMelatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the expression of genes and proteins as well as the synthesis of molecules. Among these, MT and SIRTs are valuable markers of the aging process and age-related pathologies. The purpose of this study was to examine age-related expression patterns of these signaling molecules, in particular MT, SIRT1, SIRT3, and SIRT6 in BE of subjects of different ages with and without arterial hypertension (AH). We used real-time polymerase chain reaction (RT-PCR) and immunofluorescence analysis by confocal microscopy. We found that MT immunofluorescence intensity in BE decreases with aging, more evidently in AH patients. SIRT3 and SIRT6 genes expression and immunofluorescence intensity in BE was decreased in aging controls. In AH patients, SIRT1, SIRT3, and SIRT6 gene expression and immunofluorescence intensity in BE was decreased in relation to age and in comparison with age-matched controls. In conclusion, the evaluation of MT and sirtuins in BE could provide a non-invasive method for appraising the aging process, also when accompanied by AH.https://www.mdpi.com/1422-0067/21/21/8134melatoninsirtuinsbuccal epitheliumagingarterial hypertension |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Annalucia Carbone Natalia Linkova Victoria Polyakova Ekaterina Mironova Ulduz Hashimova Ahmed Gadzhiev Khatira Safikhanova Tatiana Kvetnaia Julia Krylova Roberto Tarquini Gianluigi Mazzoccoli Igor Kvetnoy |
spellingShingle |
Annalucia Carbone Natalia Linkova Victoria Polyakova Ekaterina Mironova Ulduz Hashimova Ahmed Gadzhiev Khatira Safikhanova Tatiana Kvetnaia Julia Krylova Roberto Tarquini Gianluigi Mazzoccoli Igor Kvetnoy Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies International Journal of Molecular Sciences melatonin sirtuins buccal epithelium aging arterial hypertension |
author_facet |
Annalucia Carbone Natalia Linkova Victoria Polyakova Ekaterina Mironova Ulduz Hashimova Ahmed Gadzhiev Khatira Safikhanova Tatiana Kvetnaia Julia Krylova Roberto Tarquini Gianluigi Mazzoccoli Igor Kvetnoy |
author_sort |
Annalucia Carbone |
title |
Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies |
title_short |
Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies |
title_full |
Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies |
title_fullStr |
Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies |
title_full_unstemmed |
Melatonin and Sirtuins in Buccal Epithelium: Potential Biomarkers of Aging and Age-Related Pathologies |
title_sort |
melatonin and sirtuins in buccal epithelium: potential biomarkers of aging and age-related pathologies |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-10-01 |
description |
Melatonin (MT) and sirtuins (SIRT) are geroprotective molecules that hold back the aging process and the development of age-related diseases, including cardiovascular pathologies. Buccal epithelium (BE) sampling is a non-invasive procedure, yielding highly informative material for evaluating the expression of genes and proteins as well as the synthesis of molecules. Among these, MT and SIRTs are valuable markers of the aging process and age-related pathologies. The purpose of this study was to examine age-related expression patterns of these signaling molecules, in particular MT, SIRT1, SIRT3, and SIRT6 in BE of subjects of different ages with and without arterial hypertension (AH). We used real-time polymerase chain reaction (RT-PCR) and immunofluorescence analysis by confocal microscopy. We found that MT immunofluorescence intensity in BE decreases with aging, more evidently in AH patients. SIRT3 and SIRT6 genes expression and immunofluorescence intensity in BE was decreased in aging controls. In AH patients, SIRT1, SIRT3, and SIRT6 gene expression and immunofluorescence intensity in BE was decreased in relation to age and in comparison with age-matched controls. In conclusion, the evaluation of MT and sirtuins in BE could provide a non-invasive method for appraising the aging process, also when accompanied by AH. |
topic |
melatonin sirtuins buccal epithelium aging arterial hypertension |
url |
https://www.mdpi.com/1422-0067/21/21/8134 |
work_keys_str_mv |
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