A dot-blot assay for the low density lipoprotein receptor.
We describe a new method for detecting the interaction of low density lipoprotein with its receptor using unmodified nitrocellulose as support for membrane protein. The method is specific and sensitive down to 3 micrograms of membrane protein. Unlabeled LDL, but not HDL, competes with 125I-labeled L...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
1986-05-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520387289 |