Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019
Abstract Background Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in L...
Main Authors: | , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-05-01
|
Series: | BMC Infectious Diseases |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12879-021-06112-9 |
id |
doaj-d79852ba74eb4d3f8660c89becbc5030 |
---|---|
record_format |
Article |
spelling |
doaj-d79852ba74eb4d3f8660c89becbc50302021-05-02T11:09:10ZengBMCBMC Infectious Diseases1471-23342021-05-012111910.1186/s12879-021-06112-9Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019Jing Wang0Min Meng1Huan Xu2Ting Wang3Ying Liu4Han Yan5Peiman Liu6Daogang Qin7Qiaozhi Yang8Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalKey Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People’s HospitalAbstract Background Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng. Methods We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease. Results A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region. Conclusions This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng.https://doi.org/10.1186/s12879-021-06112-9Enterovirus (EV)Aseptic meningitisPhylogenetic analysisChildrenLiaocheng |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jing Wang Min Meng Huan Xu Ting Wang Ying Liu Han Yan Peiman Liu Daogang Qin Qiaozhi Yang |
spellingShingle |
Jing Wang Min Meng Huan Xu Ting Wang Ying Liu Han Yan Peiman Liu Daogang Qin Qiaozhi Yang Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019 BMC Infectious Diseases Enterovirus (EV) Aseptic meningitis Phylogenetic analysis Children Liaocheng |
author_facet |
Jing Wang Min Meng Huan Xu Ting Wang Ying Liu Han Yan Peiman Liu Daogang Qin Qiaozhi Yang |
author_sort |
Jing Wang |
title |
Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019 |
title_short |
Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019 |
title_full |
Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019 |
title_fullStr |
Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019 |
title_full_unstemmed |
Analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in Liaocheng, China, from 2018 to 2019 |
title_sort |
analysis of enterovirus genotypes in the cerebrospinal fluid of children associated with aseptic meningitis in liaocheng, china, from 2018 to 2019 |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2021-05-01 |
description |
Abstract Background Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng. Methods We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease. Results A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region. Conclusions This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng. |
topic |
Enterovirus (EV) Aseptic meningitis Phylogenetic analysis Children Liaocheng |
url |
https://doi.org/10.1186/s12879-021-06112-9 |
work_keys_str_mv |
AT jingwang analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT minmeng analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT huanxu analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT tingwang analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT yingliu analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT hanyan analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT peimanliu analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT daogangqin analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 AT qiaozhiyang analysisofenterovirusgenotypesinthecerebrospinalfluidofchildrenassociatedwithasepticmeningitisinliaochengchinafrom2018to2019 |
_version_ |
1721492673330675712 |