Divergent Astrovirus Associated with Neurologic Disease in Cattle
Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine enc...
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2013-09-01
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doaj-d7a9fb7008a245de9a683a53c9cdf26e2020-11-24T21:52:57ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592013-09-011991385139210.3201/eid1909.130682Divergent Astrovirus Associated with Neurologic Disease in CattleLinlin LiSantiago DiabSabrina McGrawBradd BarrRyan TraslavinaRobert HigginsTom TalbotPat BlanchardGuillermo RimoldiElizabeth FahsbenderBrady PageTung Gia PhanChunlin WangXutao DengPatricia PesaventoEric DelwartUsing viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle.https://wwwnc.cdc.gov/eid/article/19/9/13-0682_articleAstrovirusbrainneurologic diseasebovinecattlenext-generation sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Linlin Li Santiago Diab Sabrina McGraw Bradd Barr Ryan Traslavina Robert Higgins Tom Talbot Pat Blanchard Guillermo Rimoldi Elizabeth Fahsbender Brady Page Tung Gia Phan Chunlin Wang Xutao Deng Patricia Pesavento Eric Delwart |
spellingShingle |
Linlin Li Santiago Diab Sabrina McGraw Bradd Barr Ryan Traslavina Robert Higgins Tom Talbot Pat Blanchard Guillermo Rimoldi Elizabeth Fahsbender Brady Page Tung Gia Phan Chunlin Wang Xutao Deng Patricia Pesavento Eric Delwart Divergent Astrovirus Associated with Neurologic Disease in Cattle Emerging Infectious Diseases Astrovirus brain neurologic disease bovine cattle next-generation sequencing |
author_facet |
Linlin Li Santiago Diab Sabrina McGraw Bradd Barr Ryan Traslavina Robert Higgins Tom Talbot Pat Blanchard Guillermo Rimoldi Elizabeth Fahsbender Brady Page Tung Gia Phan Chunlin Wang Xutao Deng Patricia Pesavento Eric Delwart |
author_sort |
Linlin Li |
title |
Divergent Astrovirus Associated with Neurologic Disease in Cattle |
title_short |
Divergent Astrovirus Associated with Neurologic Disease in Cattle |
title_full |
Divergent Astrovirus Associated with Neurologic Disease in Cattle |
title_fullStr |
Divergent Astrovirus Associated with Neurologic Disease in Cattle |
title_full_unstemmed |
Divergent Astrovirus Associated with Neurologic Disease in Cattle |
title_sort |
divergent astrovirus associated with neurologic disease in cattle |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2013-09-01 |
description |
Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset of neurologic disease, we sequenced the complete genome of a novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In a retrospective analysis of 32 cases of bovine encephalitides of unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for viral RNA. Viral RNA was restricted to the nervous system and detected in the cytoplasm of affected neurons within the spinal cord, brainstem, and cerebellum. Microscopically, the lesions were of widespread neuronal necrosis, microgliosis, and perivascular cuffing preferentially distributed in gray matter and most severe in the cerebellum and brainstem, with increasing intensity caudally down the spinal cord. These results suggest that infection with BoAstV-NeuroS1 is a potential cause of neurologic disease in cattle. |
topic |
Astrovirus brain neurologic disease bovine cattle next-generation sequencing |
url |
https://wwwnc.cdc.gov/eid/article/19/9/13-0682_article |
work_keys_str_mv |
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