Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies
Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analys...
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doaj-d7c42a8a1ed84ce68b721ad30eb86b362020-11-24T22:27:54ZengMDPI AGMolecules1420-30492016-08-01218109210.3390/molecules21081092molecules21081092Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic TechnologiesSiu-Leung Chau0Zhi-Bing Huang1Yan-Gang Song2Rui-Qi Yue3Alan Ho4Chao-Zhan Lin5Wen-Hua Huang6Quan-Bin Han7School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, ChinaState Key Laboratory of Food Science and Technology, Sino-Germany Joint Research Institute, Nanchang University, Nanchang 330047, ChinaLimin Pharmaceutical Factory, Livzon Group, Shaoguang 512028, ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong, ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong, ChinaInstitute of Clinical Pharmacology, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, ChinaLimin Pharmaceutical Factory, Livzon Group, Shaoguang 512028, ChinaSchool of Chinese Medicine, Hong Kong Baptist University, Hong Kong, ChinaQuality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%–0.21%, and 53.49%–58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections.http://www.mdpi.com/1420-3049/21/8/1092Shenqi Fuzheng injectionChinese medicine formulaquantitative analysischemical profile |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Siu-Leung Chau Zhi-Bing Huang Yan-Gang Song Rui-Qi Yue Alan Ho Chao-Zhan Lin Wen-Hua Huang Quan-Bin Han |
spellingShingle |
Siu-Leung Chau Zhi-Bing Huang Yan-Gang Song Rui-Qi Yue Alan Ho Chao-Zhan Lin Wen-Hua Huang Quan-Bin Han Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies Molecules Shenqi Fuzheng injection Chinese medicine formula quantitative analysis chemical profile |
author_facet |
Siu-Leung Chau Zhi-Bing Huang Yan-Gang Song Rui-Qi Yue Alan Ho Chao-Zhan Lin Wen-Hua Huang Quan-Bin Han |
author_sort |
Siu-Leung Chau |
title |
Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies |
title_short |
Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies |
title_full |
Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies |
title_fullStr |
Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies |
title_full_unstemmed |
Comprehensive Quantitative Analysis of SQ Injection Using Multiple Chromatographic Technologies |
title_sort |
comprehensive quantitative analysis of sq injection using multiple chromatographic technologies |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-08-01 |
description |
Quality control of Chinese medicine injections remains a challenge due to our poor knowledge of their complex chemical profile. This study aims to investigate the chemical composition of one of the best-selling injections, Shenqi Fuzheng (SQ) injection (SQI), via a full component quantitative analysis. A total of 15 representative small molecular components of SQI were simultaneously determined using ultra-high performance liquid chromatography (UHPLC) coupled with quadrupole tandem time-of-flight mass spectrometry (Q-TOF-MS); saccharide composition of SQI was also quantitatively determined by high performance liquid chromatography (HPLC) with evaporative light scattering detector (ELSD) on an amino column before and after acid hydrolysis. The existence of polysaccharides was also examined on a gel permeation chromatography column. The method was well validated in terms of linearity, sensitivity, precision, accuracy and stability, and was successfully applied to analyze 13 SQI samples. The results demonstrate that up to 94.69% (w/w) of this injection product are quantitatively determined, in which small molecules and monosaccharide/sucrose account for 0.18%–0.21%, and 53.49%–58.2%, respectively. The quantitative information contributes to accumulating scientific evidence to better understand the therapy efficacy and safety of complex Chinese medicine injections. |
topic |
Shenqi Fuzheng injection Chinese medicine formula quantitative analysis chemical profile |
url |
http://www.mdpi.com/1420-3049/21/8/1092 |
work_keys_str_mv |
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