Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil
Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsa...
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doaj-d7d5c83429564a1da1c204cd9ebeca342020-11-24T22:16:31ZengMDPI AGMolecules1420-30492017-10-012210177110.3390/molecules22101771molecules22101771Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver OilAnna Chojnacka0Witold Gładkowski1Aleksandra Grudniewska2Department of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, PolandDepartment of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, PolandDepartment of Chemistry, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, PolandPhospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as the natural acyl donors. PUFA-EF was produced by urea-complexation and contained 86.9% PUFA including 8.5% stearidonic acid (SDA; 18:4(n-3)), 26.7% EPA, and 45.2% DHA. The transesterification of PC with PUFA was catalyzed by lipases. After screening of enzymes the effect of reaction medium; molar ratio of substrates and etc. was investigated. The highest incorporation of PUFA was 45.6%; including 36.8% DHA and 5.8% EPA at the following reaction conditions: hexane; 55 °C; PUFA-EF/PC acyl ratio of 10; 48 h of reaction time and lipase B from Candida antarctica as a biocatalyst (20% of enzyme load).https://www.mdpi.com/1420-3049/22/10/1771PUFA-enriched phospholipidslipasesphospholipids transesterificationn-3 PUFA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Anna Chojnacka Witold Gładkowski Aleksandra Grudniewska |
spellingShingle |
Anna Chojnacka Witold Gładkowski Aleksandra Grudniewska Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil Molecules PUFA-enriched phospholipids lipases phospholipids transesterification n-3 PUFA |
author_facet |
Anna Chojnacka Witold Gładkowski Aleksandra Grudniewska |
author_sort |
Anna Chojnacka |
title |
Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil |
title_short |
Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil |
title_full |
Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil |
title_fullStr |
Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil |
title_full_unstemmed |
Lipase-Catalyzed Transesterification of Egg-Yolk Phophatidylcholine with Concentrate of n-3 Polyunsaturated Fatty Acids from Cod Liver Oil |
title_sort |
lipase-catalyzed transesterification of egg-yolk phophatidylcholine with concentrate of n-3 polyunsaturated fatty acids from cod liver oil |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2017-10-01 |
description |
Phospholipids containing PUFAs are important vehicles for their delivering to the targeted tissues. In our research project we established enzymatic methods for the enrichment of natural egg-yolk PC with n-3 PUFAs. Instead of synthetic PUFA ethyl esters, the new strategy was developed using polyunsaturated fatty acids enriched fraction (PUFA-EF) from cod liver oil as the natural acyl donors. PUFA-EF was produced by urea-complexation and contained 86.9% PUFA including 8.5% stearidonic acid (SDA; 18:4(n-3)), 26.7% EPA, and 45.2% DHA. The transesterification of PC with PUFA was catalyzed by lipases. After screening of enzymes the effect of reaction medium; molar ratio of substrates and etc. was investigated. The highest incorporation of PUFA was 45.6%; including 36.8% DHA and 5.8% EPA at the following reaction conditions: hexane; 55 °C; PUFA-EF/PC acyl ratio of 10; 48 h of reaction time and lipase B from Candida antarctica as a biocatalyst (20% of enzyme load). |
topic |
PUFA-enriched phospholipids lipases phospholipids transesterification n-3 PUFA |
url |
https://www.mdpi.com/1420-3049/22/10/1771 |
work_keys_str_mv |
AT annachojnacka lipasecatalyzedtransesterificationofeggyolkphophatidylcholinewithconcentrateofn3polyunsaturatedfattyacidsfromcodliveroil AT witoldgładkowski lipasecatalyzedtransesterificationofeggyolkphophatidylcholinewithconcentrateofn3polyunsaturatedfattyacidsfromcodliveroil AT aleksandragrudniewska lipasecatalyzedtransesterificationofeggyolkphophatidylcholinewithconcentrateofn3polyunsaturatedfattyacidsfromcodliveroil |
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