Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery
This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sar...
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doaj-d7f31642d392441ca1aa96c8894a86b12020-11-24T21:02:26ZengMDPI AGNanomaterials2079-49912017-12-0171243510.3390/nano7120435nano7120435Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer DeliverySylvie Skalickova0Martin Loffelmann1Michael Gargulak2Marta Kepinska3Michaela Docekalova4Dagmar Uhlirova5Martina Stankova6Carlos Fernandez7Halina Milnerowicz8Branislav Ruttkay-Nedecky9Rene Kizek10Department of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicCentral Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicCentral Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicFaculty of Pharmacy, Department of Biomedical and Environmental Analyses, Wroclaw Medical University, 50-556 Wrocław, PolandCentral Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicCentral Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicCentral Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicSchool of Pharmacy and Life Sciences, Robert Gordon University, Garthdee Road, Aberdeen AB10 7QB, UKFaculty of Pharmacy, Department of Biomedical and Environmental Analyses, Wroclaw Medical University, 50-556 Wrocław, PolandCentral Laboratory, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicDepartment of Human Pharmacology and Toxicology, Faculty of Pharmacy, University of Veterinary and Pharmaceutical Sciences Brno, 61200 Brno, Czech RepublicThis work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from −960 to −950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin and strong dependence of drug release after 120 min in the blood environment. The functionality of the designed nanotransporter was proven. The purposed determination showed linear dependence in the concentration range of Anti-sarcosine IgG labeled gold nanoparticles from 0 to 1000 µg/mL and the regression equation was found to be y = 3.8x − 66.7 and R2 = 0.99. Performed ELISA confirmed the ability of Anti-sarcosine IgG labeled chitosan nanoparticles with loaded doxorubicin to bind to the sarcosine molecule. Observed hemolytic activity of the nanotransporter was 40%. Inhibition activity of our proposed nanotransporter was evaluated to be 0% on the experimental model of S. cerevisiae. Anti-sarcosine IgG labeled chitosan nanoparticles, with loaded doxorubicin stabilized by Zn ions, are a perspective type of nanocarrier for targeted drug therapy managed by specific interaction with sarcosine and metallothionein for prostate cancer.https://www.mdpi.com/2079-4991/7/12/435doxorubicinchitosanzincsarcosineninhydrinprostate cancerperoxidase activitygold nanoparticlemagnetic gold nanoparticle |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sylvie Skalickova Martin Loffelmann Michael Gargulak Marta Kepinska Michaela Docekalova Dagmar Uhlirova Martina Stankova Carlos Fernandez Halina Milnerowicz Branislav Ruttkay-Nedecky Rene Kizek |
spellingShingle |
Sylvie Skalickova Martin Loffelmann Michael Gargulak Marta Kepinska Michaela Docekalova Dagmar Uhlirova Martina Stankova Carlos Fernandez Halina Milnerowicz Branislav Ruttkay-Nedecky Rene Kizek Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery Nanomaterials doxorubicin chitosan zinc sarcosine ninhydrin prostate cancer peroxidase activity gold nanoparticle magnetic gold nanoparticle |
author_facet |
Sylvie Skalickova Martin Loffelmann Michael Gargulak Marta Kepinska Michaela Docekalova Dagmar Uhlirova Martina Stankova Carlos Fernandez Halina Milnerowicz Branislav Ruttkay-Nedecky Rene Kizek |
author_sort |
Sylvie Skalickova |
title |
Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery |
title_short |
Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery |
title_full |
Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery |
title_fullStr |
Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery |
title_full_unstemmed |
Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery |
title_sort |
zinc-modified nanotransporter of doxorubicin for targeted prostate cancer delivery |
publisher |
MDPI AG |
series |
Nanomaterials |
issn |
2079-4991 |
publishDate |
2017-12-01 |
description |
This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from −960 to −950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin and strong dependence of drug release after 120 min in the blood environment. The functionality of the designed nanotransporter was proven. The purposed determination showed linear dependence in the concentration range of Anti-sarcosine IgG labeled gold nanoparticles from 0 to 1000 µg/mL and the regression equation was found to be y = 3.8x − 66.7 and R2 = 0.99. Performed ELISA confirmed the ability of Anti-sarcosine IgG labeled chitosan nanoparticles with loaded doxorubicin to bind to the sarcosine molecule. Observed hemolytic activity of the nanotransporter was 40%. Inhibition activity of our proposed nanotransporter was evaluated to be 0% on the experimental model of S. cerevisiae. Anti-sarcosine IgG labeled chitosan nanoparticles, with loaded doxorubicin stabilized by Zn ions, are a perspective type of nanocarrier for targeted drug therapy managed by specific interaction with sarcosine and metallothionein for prostate cancer. |
topic |
doxorubicin chitosan zinc sarcosine ninhydrin prostate cancer peroxidase activity gold nanoparticle magnetic gold nanoparticle |
url |
https://www.mdpi.com/2079-4991/7/12/435 |
work_keys_str_mv |
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