Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India
ABSTRACT Background and Objective: Urinary Tract Infections (UTIs) are mostly caused by Escherichia coli. The appropriate therapy demands a current knowledge on the antimicrobial susceptibility pattern amongst these pathogens, as an inappropriate use of antibiotics may lead to complications and...
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doaj-d82135e7570243bca9a6318eacd5bcbc2020-11-25T03:15:44ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2013-03-017344945310.7860/JCDR/2013/4990.2796Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, IndiaMandira Mukherjee0Shreya Basu1Sandip Kumar Mukherjee2Monalisa Majumder3Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata, West Bengal, IndiaDepartment of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata, West Bengal, India.Department of Biochemistry and Medical Biotechnology, Calcutta School of Tropical Medicine, Kolkata, West Bengal, India.Department of Microbiology, Bacteriology and Serology Division, Calcutta School of Tropical Medicine, Kolkata, West Bengal, India.ABSTRACT Background and Objective: Urinary Tract Infections (UTIs) are mostly caused by Escherichia coli. The appropriate therapy demands a current knowledge on the antimicrobial susceptibility pattern amongst these pathogens, as an inappropriate use of antibiotics may lead to complications and treatment failure. The UTIs which are caused by multidrug resistant ExtendedSpectrum Beta-Lactamase (ESBL) producing bacteria further pose a severe problem, as the treatment options are limited. The aim of this study was to identify the pattern of multi drug resistance amongst the uropathogenic E. coli (UPEC) isolates which were obtained from hospitalized patients. Materials and Methods: Forty UPEC were isolated from 200 urine samples of hospitalized patients who were clinically suspected for UTIs. Antimicrobial susceptibility screening was performed by using 16 antibiotics, by the Kirby Bauer disk diffusion technique. The isolates which were resistant to the third generation cephalosporins were subjected to the ESBL confirmatory test by using drug and drug-inhibitor combination disks by following the CLSI guidelines. Results: All the 40 isolates except three were multidrug resistant. They showed the highest sensitivities for nitrofurantoin (72.5%) and amikacin (70%). A high level of resistance was observed against ampicillin (97.5%), nalidixic acid and cefelexin (95%), amoxicillin (92.5%), cotrimoxazole (82.5%) and ciprofloxacin (80%) respectively. Thirty different antibiotic resistance patterns were observed against the different antibiotics. Twenty-eight out of the 40 isolates were resistant to the third generation cephalosporins. However, the phenotypic test for the ESBL confirmation indicated that eighteen out of the twenty-eight isolates were ESBL producers and that eleven different drug resistance patterns were observed amongst them. Conclusions: Therefore, this study accounts for the varied multidrug resistance pattern amongst the uropathogenic E. coli which were isolated from hospitalized patients in Kolkata, an eastern region of India. Nitrofurantoin and amikacin should be assigned as potent drugs to treat this infection in this region of the country. These varied resistance patterns present major therapeutic and infection control challenges and they suggest a heterogeneous population of the uropathogenic E. coli isolates which circulate in this sector of India. https://www.jcdr.net/articles/PDF/2796/10-%204990_u.pdfantibiotic susceptibilityurinary tract infectionuropathogenic e. coliextended- spectrum beta lactamase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mandira Mukherjee Shreya Basu Sandip Kumar Mukherjee Monalisa Majumder |
spellingShingle |
Mandira Mukherjee Shreya Basu Sandip Kumar Mukherjee Monalisa Majumder Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India Journal of Clinical and Diagnostic Research antibiotic susceptibility urinary tract infection uropathogenic e. coli extended- spectrum beta lactamase |
author_facet |
Mandira Mukherjee Shreya Basu Sandip Kumar Mukherjee Monalisa Majumder |
author_sort |
Mandira Mukherjee |
title |
Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India |
title_short |
Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India |
title_full |
Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India |
title_fullStr |
Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India |
title_full_unstemmed |
Multidrug-Resistance and Extended Spectrum Beta-Lactamase Production in Uropathogenic E. Coli which were Isolated from Hospitalized Patients in Kolkata, India |
title_sort |
multidrug-resistance and extended spectrum beta-lactamase production in uropathogenic e. coli which were isolated from hospitalized patients in kolkata, india |
publisher |
JCDR Research and Publications Private Limited |
series |
Journal of Clinical and Diagnostic Research |
issn |
2249-782X 0973-709X |
publishDate |
2013-03-01 |
description |
ABSTRACT
Background and Objective: Urinary Tract Infections (UTIs)
are mostly caused by Escherichia coli. The appropriate therapy
demands a current knowledge on the antimicrobial susceptibility
pattern amongst these pathogens, as an inappropriate use of
antibiotics may lead to complications and treatment failure.
The UTIs which are caused by multidrug resistant ExtendedSpectrum Beta-Lactamase (ESBL) producing bacteria further
pose a severe problem, as the treatment options are limited.
The aim of this study was to identify the pattern of multi drug
resistance amongst the uropathogenic E. coli (UPEC) isolates
which were obtained from hospitalized patients.
Materials and Methods: Forty UPEC were isolated from 200
urine samples of hospitalized patients who were clinically
suspected for UTIs. Antimicrobial susceptibility screening was
performed by using 16 antibiotics, by the Kirby Bauer disk
diffusion technique. The isolates which were resistant to the
third generation cephalosporins were subjected to the ESBL
confirmatory test by using drug and drug-inhibitor combination
disks by following the CLSI guidelines.
Results: All the 40 isolates except three were multidrug
resistant. They showed the highest sensitivities for nitrofurantoin
(72.5%) and amikacin (70%). A high level of resistance
was observed against ampicillin (97.5%), nalidixic acid and
cefelexin (95%), amoxicillin (92.5%), cotrimoxazole (82.5%)
and ciprofloxacin (80%) respectively. Thirty different antibiotic
resistance patterns were observed against the different
antibiotics. Twenty-eight out of the 40 isolates were resistant to
the third generation cephalosporins. However, the phenotypic
test for the ESBL confirmation indicated that eighteen out of
the twenty-eight isolates were ESBL producers and that eleven
different drug resistance patterns were observed amongst
them.
Conclusions: Therefore, this study accounts for the varied
multidrug resistance pattern amongst the uropathogenic E. coli
which were isolated from hospitalized patients in Kolkata, an
eastern region of India. Nitrofurantoin and amikacin should be
assigned as potent drugs to treat this infection in this region
of the country. These varied resistance patterns present major
therapeutic and infection control challenges and they suggest a
heterogeneous population of the uropathogenic E. coli isolates
which circulate in this sector of India.
|
topic |
antibiotic susceptibility urinary tract infection uropathogenic e. coli extended- spectrum beta lactamase |
url |
https://www.jcdr.net/articles/PDF/2796/10-%204990_u.pdf |
work_keys_str_mv |
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