Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified Ganoderma lucidum strain QRS 5120 using response surface methodology

Wild-cultivated medicinal mushroom Ganoderma lucidum was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified ...

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Main Authors:	Sugenendran Supramani, Rahayu Ahmad, Zul Ilham, Mohamad Suffian Mohamad Annuar, Anita Klaus, Wan Abd Al Qadr Imad Wan-Mohtar
Format:	Article
Language:	English
Published:	AIMS Press 2019-01-01
Series:	AIMS Microbiology
Subjects:	<em>ganoderma lucidum</em> response surface methodology submerged-liquid fermentation exopolysaccharide intracellular polysaccharide
Online Access:	https://www.aimspress.com/article/10.3934/microbiol.2019.1.19/fulltext.html

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spelling	doaj-d8297aea748345e5981eaa44326e94c22020-11-25T02:07:40ZengAIMS PressAIMS Microbiology2471-18882019-01-0151193810.3934/microbiol.2019.1.19Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodologySugenendran Supramani0Rahayu Ahmad1Zul Ilham2 Mohamad Suffian Mohamad Annuar3Anita Klaus4Wan Abd Al Qadr Imad Wan-Mohtar51 Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia2 Halal Action Laboratory, Kolej Permata Insan, University Sains Islam Malaysia, Bandar Baru Nilai, 71800, Nilai, Negeri Sembilan, Malaysia3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia3 Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia4 Institute for Food Technology and Biochemistry, Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080 Belgrade, Serbia1 Functional Omics and Bioprocess Development Laboratory, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, MalaysiaWild-cultivated medicinal mushroom <em>Ganoderma lucidum</em> was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified <em>G. lucidum</em> was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the <em>G. lucidum</em> strain QRS 5120 generated 637 base pairs, which was commensurate with related <em>Ganoderma</em> species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (<em>p</em> < 0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (<em>p</em> < 0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using <em>G. lucidum</em>.https://www.aimspress.com/article/10.3934/microbiol.2019.1.19/fulltext.html<em>ganoderma lucidum</em>response surface methodologysubmerged-liquid fermentationexopolysaccharideintracellular polysaccharide
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sugenendran Supramani Rahayu Ahmad Zul Ilham Mohamad Suffian Mohamad Annuar Anita Klaus Wan Abd Al Qadr Imad Wan-Mohtar
spellingShingle	Sugenendran Supramani Rahayu Ahmad Zul Ilham Mohamad Suffian Mohamad Annuar Anita Klaus Wan Abd Al Qadr Imad Wan-Mohtar Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology AIMS Microbiology <em>ganoderma lucidum</em> response surface methodology submerged-liquid fermentation exopolysaccharide intracellular polysaccharide
author_facet	Sugenendran Supramani Rahayu Ahmad Zul Ilham Mohamad Suffian Mohamad Annuar Anita Klaus Wan Abd Al Qadr Imad Wan-Mohtar
author_sort	Sugenendran Supramani
title	Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology
title_short	Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology
title_full	Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology
title_fullStr	Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology
title_full_unstemmed	Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology
title_sort	optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>ganoderma lucidum</em> strain qrs 5120 using response surface methodology
publisher	AIMS Press
series	AIMS Microbiology
issn	2471-1888
publishDate	2019-01-01
description	Wild-cultivated medicinal mushroom <em>Ganoderma lucidum</em> was morphologically identified and sequenced using phylogenetic software. In submerged-liquid fermentation (SLF), biomass, exopolysaccharide (EPS) and intracellular polysaccharide (IPS) production of the identified <em>G. lucidum</em> was optimised based on initial pH, starting glucose concentration and agitation rate parameters using response surface methodology (RSM). Molecularly, the <em>G. lucidum</em> strain QRS 5120 generated 637 base pairs, which was commensurate with related <em>Ganoderma</em> species. In RSM, by applying central composite design (CCD), a polynomial model was fitted to the experimental data and was found to be significant in all parameters investigated. The strongest effect (<em>p</em> < 0.0001) was observed for initial pH for biomass, EPS and IPS production, while agitation showed a significant value (<em>p</em> < 0.005) for biomass. By applying the optimized conditions, the model was validated and generated 5.12 g/L of biomass (initial pH 4.01, 32.09 g/L of glucose and 102 rpm), 2.49 g/L EPS (initial pH 4, 24.25 g/L of glucose and 110 rpm) and 1.52 g/L of IPS (and initial pH 4, 40.43 g/L of glucose, 103 rpm) in 500 mL shake flask fermentation. The optimized parameters can be upscaled for efficient biomass, EPS and IPS production using <em>G. lucidum</em>.
topic	<em>ganoderma lucidum</em> response surface methodology submerged-liquid fermentation exopolysaccharide intracellular polysaccharide
url	https://www.aimspress.com/article/10.3934/microbiol.2019.1.19/fulltext.html
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Optimisation of biomass, exopolysaccharide and intracellular polysaccharide production from the mycelium of an identified <em>Ganoderma lucidum</em> strain QRS 5120 using response surface methodology

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