Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling
Abstract Background Activation of Adenosine 5′-monophosphate-activated protein kinase/Sirtuin1 (AMPK/SIRT1) exerts an effect in alleviating obesity and gut damage. Sodium nitroprusside (SNP), a nitric oxide (NO) donor, has been reported to activate AMPK. This study was to investigate the effect of S...
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doaj-d837bd80dad44c92b8d789534b8a94272021-10-03T11:21:55ZengBMCBMC Gastroenterology1471-230X2021-10-0121111110.1186/s12876-021-01934-ySodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signalingXiaomei Li0Chen Li1Yuanqi Li2Cong Liu3Xue Liang4Ting Liu5Zhihua Liu6Guangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityGuangzhou Key Laboratory of Enhanced Recovery after Abdominal Surgery, Innovation Center for Advanced Interdisciplinary Medicine, The Fifth Affiliated Hospital of Guangzhou Medical UniversityAbstract Background Activation of Adenosine 5′-monophosphate-activated protein kinase/Sirtuin1 (AMPK/SIRT1) exerts an effect in alleviating obesity and gut damage. Sodium nitroprusside (SNP), a nitric oxide (NO) donor, has been reported to activate AMPK. This study was to investigate the effect of SNP on HFD induced gut dysfunction and the mechanism. Methods SNP was applied on lipopolysaccharide (LPS) stimulated Caco-2 cell monolayers which mimicked intestinal epithelial barrier dysfunction and HFD-fed mice which were complicated by gut dysfunction. Then AMPKα/SIRT1 pathway and gut barrier indicators were investigated. Results SNP rescued the loss of tight junction proteins ZO-1 and occludin, the inhibition of AMPKα/SIRT1 in LPS stimulated Caco-2 cell monolayers, and the effects were not shown when AMPKa1 was knocked-down by siRNA. SNP also alleviated HFD induced obesity and gut dysfunction in mice, as indicated by the decreasing of intestinal permeability, the increasing expression of ZO-1 and occludin, the decreasing levels of pro-inflammatory cytokine IL-6, and the repairing of gut microbiota dysbiosis. These effects were complicated by the increased colonic NO content and the activated AMPKα/SIRT1 signaling. Conclusions The results may imply that SNP, as a NO donor, alleviates HFD induced gut dysfunction probably by activating the AMPKα/SIRT1 signaling pathway.https://doi.org/10.1186/s12876-021-01934-yAMPKα/SIRT1 signalingNOSNPIntestinal barrier dysfunctionHFDGut microbiota dysbiosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xiaomei Li Chen Li Yuanqi Li Cong Liu Xue Liang Ting Liu Zhihua Liu |
spellingShingle |
Xiaomei Li Chen Li Yuanqi Li Cong Liu Xue Liang Ting Liu Zhihua Liu Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling BMC Gastroenterology AMPKα/SIRT1 signaling NO SNP Intestinal barrier dysfunction HFD Gut microbiota dysbiosis |
author_facet |
Xiaomei Li Chen Li Yuanqi Li Cong Liu Xue Liang Ting Liu Zhihua Liu |
author_sort |
Xiaomei Li |
title |
Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling |
title_short |
Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling |
title_full |
Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling |
title_fullStr |
Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling |
title_full_unstemmed |
Sodium nitroprusside protects HFD induced gut dysfunction via activating AMPKα/SIRT1 signaling |
title_sort |
sodium nitroprusside protects hfd induced gut dysfunction via activating ampkα/sirt1 signaling |
publisher |
BMC |
series |
BMC Gastroenterology |
issn |
1471-230X |
publishDate |
2021-10-01 |
description |
Abstract Background Activation of Adenosine 5′-monophosphate-activated protein kinase/Sirtuin1 (AMPK/SIRT1) exerts an effect in alleviating obesity and gut damage. Sodium nitroprusside (SNP), a nitric oxide (NO) donor, has been reported to activate AMPK. This study was to investigate the effect of SNP on HFD induced gut dysfunction and the mechanism. Methods SNP was applied on lipopolysaccharide (LPS) stimulated Caco-2 cell monolayers which mimicked intestinal epithelial barrier dysfunction and HFD-fed mice which were complicated by gut dysfunction. Then AMPKα/SIRT1 pathway and gut barrier indicators were investigated. Results SNP rescued the loss of tight junction proteins ZO-1 and occludin, the inhibition of AMPKα/SIRT1 in LPS stimulated Caco-2 cell monolayers, and the effects were not shown when AMPKa1 was knocked-down by siRNA. SNP also alleviated HFD induced obesity and gut dysfunction in mice, as indicated by the decreasing of intestinal permeability, the increasing expression of ZO-1 and occludin, the decreasing levels of pro-inflammatory cytokine IL-6, and the repairing of gut microbiota dysbiosis. These effects were complicated by the increased colonic NO content and the activated AMPKα/SIRT1 signaling. Conclusions The results may imply that SNP, as a NO donor, alleviates HFD induced gut dysfunction probably by activating the AMPKα/SIRT1 signaling pathway. |
topic |
AMPKα/SIRT1 signaling NO SNP Intestinal barrier dysfunction HFD Gut microbiota dysbiosis |
url |
https://doi.org/10.1186/s12876-021-01934-y |
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