Homoisoflavonoids and Chalcones Isolated from Haematoxylum campechianum L., with Spasmolytic Activity

• Main Authors: Armando Escobar-Ramos, Carlos Ernesto Lobato-García, Alejandro Zamilpa, Abraham Gómez-Rivera, Jaime Tortoriello, Manasés González-Cortazar
• Format: Article
• Language: English
• Published: MDPI AG 2017-08-01
• Series: Molecules
• Subjects: homoisoflavonoids; Haematoxylum campechianum; chalcone; spasmolytic
• Online Access: https://www.mdpi.com/1420-3049/22/9/1405

Haematoxylum campechianum is a medicinal plant employed as an astringent to purify the blood and to treat stomach problems such as diarrhea and dysentery. A bio-guided chemical fractionation of the methanolic extract obtained from this plant allowed for the isolation of five compounds: two chalcones known as sappanchalcone (1); 3-deoxysappanchalcone (2); three homoisoflavonoids known as hematoxylol A (3); 4-O-methylhematoxylol (4); and, hematoxin (5). The spasmolytic activity was determined in an in vitro model (electrically induced contractions of guinea pig ileum), and allowed to demonstrate that the methanolic extract (EC50 = 62.11 ± 3.23) fractions HcF7 (EC50 = 61.75 ± 3.55) and HcF9 (EC50 = 125.5 ± 10.65) and compounds 1 (EC50 = 16.06 ± 2.15) and 2 (EC50 = 25.37 ± 3.47) of Haematoxylum campechianum present significant relaxing activity as compared to papaverine (EC50 = 20.08 ± 2.0) as a positive control.