Potentialities of use of affinity interaction of biologically active substances in practice of public health system

• Main Authors: V I Pokrovsky, V V Maleev, А В Zborovsky, V I Efremenko, I P Gontar, I S Tyumentseva, Ye N Afanasyev
• Format: Article
• Language: Russian
• Published: "Consilium Medicum" Publishing house 2003-11-01
• Series: Терапевтический архив
• Subjects: magnetic immunosorbents; biologically active substances; particularly dangerous and other infections; rheumatic diseases
• Online Access: https://ter-arkhiv.ru/0040-3660/article/view/29700

Aim. To determine the potentialities of use of affinity interaction of immobilized biologically active substances (bacterial cells or their fragments, toxins, antigens of various chemical nature, immunoglobulins, enzymes, gangliosides, etc.) for medical practice. Materials and methods. Emulsion polymerization of acrylamide monomers in the gaseous nitrogen current was used as a basic method for preparation of solid-phase magnetic immunosorbents (MIC). A procedure for preparation of siliceous MIC was also applied. The prepared MICs were used a solid phase in enzyme immunoassay and immunofluorescence assay and the recorded data were compared with those of stidied conventionally used in practical medicine. Results. The use of MIC made it possible to detect pathogens of particularly dangerous infections in large volumes of the samples contaminated with another microflora. With the proposed MIC, one can stand a good chance of surveying large contingents of the population, of obtaining the quantitative results in shorter periods to establish a diagnosis. With this, the sensitivity and specificity of immunoassays substantially increase. Whether MIC may be used as selective hemosorbents to remove specific antibodies from the blood of patients with rheumatic diseases for therapeutic purposes was studied. Conclusion. The findings are indicative of wide potentialities of use of affinity interaction of biologically active substances immobilized on inert carriers with the inserted magnetic material in the laboratory diagnosis of diseases of both infectious and autoimmune nature, which may be widely used in the inand outpatient settings.