Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach
Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range of metabolites, including gl...
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doaj-d85775ec51e24e718c5d232937c6777b2020-11-24T20:46:04ZengElsevierJournal of Food and Drug Analysis1021-94982015-09-0123343344110.1016/j.jfda.2015.01.005Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approachNeda Javadi0Faridah Abas1Ahmed Mediani2Azizah Abd Hamid3Alfi Khatib4Sanimah Simoh5Khozirah Shaari6Department of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 Serdang, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 Serdang, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 Serdang, MalaysiaDepartment of Food Science, Faculty of Food Science and Technology, University Putra Malaysia, 43400 Serdang, MalaysiaDepartment of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, MalaysiaBiotechnology Research Centre, MARDI Headquarters, Serdang, P.O. Box 12301, 50744, Kuala Lumpur, MalaysiaLaboratory of Natural Products, Institute of Bioscience, University Putra Malaysia, 43400 Serdang, MalaysiaCosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range of metabolites, including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of these metabolites are not stable and easily degraded or modified during storage. In order to investigate the metabolomics changes occurring during post-harvest storage, C. caudatus samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, and 12 hours) at room temperature. As the model experiment, the metabolites identified by gas chromatography-mass spectrometry (GC-MS) were correlated with α-glucosidase inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the variation among samples and metabolites contributing to the activity. Orthogonal partial least squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound chemical alteration, both in primary and secondary metabolites, was observed. The α-tocopherol, catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and lycopene compounds were found to be the discriminating metabolites at early storage; however, sugars such as sucrose, α-d-galactopyranose, and turanose were detected, which was attributed to the discriminating metabolites for late storage. The result shows that the MVDA method is a promising technique to identify biomarker compounds relative to storage at different times.http://www.sciencedirect.com/science/article/pii/S1021949815000332Cosmos caudatusGC-MSMVDAOPLSstorage |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Neda Javadi Faridah Abas Ahmed Mediani Azizah Abd Hamid Alfi Khatib Sanimah Simoh Khozirah Shaari |
spellingShingle |
Neda Javadi Faridah Abas Ahmed Mediani Azizah Abd Hamid Alfi Khatib Sanimah Simoh Khozirah Shaari Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach Journal of Food and Drug Analysis Cosmos caudatus GC-MS MVDA OPLS storage |
author_facet |
Neda Javadi Faridah Abas Ahmed Mediani Azizah Abd Hamid Alfi Khatib Sanimah Simoh Khozirah Shaari |
author_sort |
Neda Javadi |
title |
Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach |
title_short |
Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach |
title_full |
Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach |
title_fullStr |
Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach |
title_full_unstemmed |
Effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of Cosmos caudatus leaves – GCMS based metabolomics approach |
title_sort |
effect of storage time on metabolite profile and alpha-glucosidase inhibitory activity of cosmos caudatus leaves – gcms based metabolomics approach |
publisher |
Elsevier |
series |
Journal of Food and Drug Analysis |
issn |
1021-9498 |
publishDate |
2015-09-01 |
description |
Cosmos caudatus, which is a commonly consumed vegetable in Malaysia, is locally known as “Ulam Raja”. It is a local Malaysian herb traditionally used as a food and medicinal herb to treat several maladies. Its bioactive or nutritional constituents consist of a wide range of metabolites, including glucosinolates, phenolics, amino acids, organic acids, and sugars. However, many of these metabolites are not stable and easily degraded or modified during storage. In order to investigate the metabolomics changes occurring during post-harvest storage, C. caudatus samples were subjected to seven different storage times (0 hours, 2 hours, 4 hours, 6 hours, 8 hours, 10 hours, and 12 hours) at room temperature. As the model experiment, the metabolites identified by gas chromatography-mass spectrometry (GC-MS) were correlated with α-glucosidase inhibitory activity analyzed with multivariate data analysis (MVDA) to find out the variation among samples and metabolites contributing to the activity. Orthogonal partial least squares (OPLS) analysis was applied to investigate the metabolomics changes. A profound chemical alteration, both in primary and secondary metabolites, was observed. The α-tocopherol, catechin, cyclohexen-1-carboxylic acid, benzoic acid, myo-inositol, stigmasterol, and lycopene compounds were found to be the discriminating metabolites at early storage; however, sugars such as sucrose, α-d-galactopyranose, and turanose were detected, which was attributed to the discriminating metabolites for late storage. The result shows that the MVDA method is a promising technique to identify biomarker compounds relative to storage at different times. |
topic |
Cosmos caudatus GC-MS MVDA OPLS storage |
url |
http://www.sciencedirect.com/science/article/pii/S1021949815000332 |
work_keys_str_mv |
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