Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor
Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findi...
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doaj-d85e6dcd036843939fa82fdcb4d1240b2020-11-24T21:48:59ZengHindawi LimitedThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/962083962083Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter BioreactorHamid Reza Fard Masoumi0Mahiran Basri1Anuar Kassim2Dzulkefly Kuang Abdullah3Yadollah Abdollahi4Siti Salwa Abd Gani5Malahat Rezaee6Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaMaterial Synthesis and Characterization Laboratory, Institute of Advanced Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaInstitute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, MalaysiaLipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process.http://dx.doi.org/10.1155/2013/962083 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hamid Reza Fard Masoumi Mahiran Basri Anuar Kassim Dzulkefly Kuang Abdullah Yadollah Abdollahi Siti Salwa Abd Gani Malahat Rezaee |
spellingShingle |
Hamid Reza Fard Masoumi Mahiran Basri Anuar Kassim Dzulkefly Kuang Abdullah Yadollah Abdollahi Siti Salwa Abd Gani Malahat Rezaee Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor The Scientific World Journal |
author_facet |
Hamid Reza Fard Masoumi Mahiran Basri Anuar Kassim Dzulkefly Kuang Abdullah Yadollah Abdollahi Siti Salwa Abd Gani Malahat Rezaee |
author_sort |
Hamid Reza Fard Masoumi |
title |
Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor |
title_short |
Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor |
title_full |
Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor |
title_fullStr |
Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor |
title_full_unstemmed |
Statistical Optimization of Process Parameters for Lipase-Catalyzed Synthesis of Triethanolamine-Based Esterquats Using Response Surface Methodology in 2-Liter Bioreactor |
title_sort |
statistical optimization of process parameters for lipase-catalyzed synthesis of triethanolamine-based esterquats using response surface methodology in 2-liter bioreactor |
publisher |
Hindawi Limited |
series |
The Scientific World Journal |
issn |
1537-744X |
publishDate |
2013-01-01 |
description |
Lipase-catalyzed production of triethanolamine-based esterquat by esterification of oleic acid (OA) with triethanolamine (TEA) in n-hexane was performed in 2 L stirred-tank reactor. A set of experiments was designed by central composite design to process modeling and statistically evaluate the findings. Five independent process variables, including enzyme amount, reaction time, reaction temperature, substrates molar ratio of OA to TEA, and agitation speed, were studied under the given conditions designed by Design Expert software. Experimental data were examined for normality test before data processing stage and skewness and kurtosis indices were determined. The mathematical model developed was found to be adequate and statistically accurate to predict the optimum conversion of product. Response surface methodology with central composite design gave the best performance in this study, and the methodology as a whole has been proven to be adequate for the design and optimization of the enzymatic process. |
url |
http://dx.doi.org/10.1155/2013/962083 |
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