Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein
<p>Abstract</p> <p>Background</p> <p>The interaction of human T-cell leukemia virus type 1 (HTLV-1) Tax1 protein with the tumor suppressor Dlg1 is correlated with cellular transformation.</p> <p>Results</p> <p>Here, we show that Dlg1 knockdown by...
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doaj-d8a93f5fc4c549128af4ae2f7293ac092020-11-24T21:33:52ZengBMCRetrovirology1742-46902006-10-01317110.1186/1742-4690-3-71Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax proteinTanaka YuetsuOie MasayasuYoshida SakikoTakahashi MasahikoHiguchi MasayaIshioka KojiroTakahashi SugataXie LiGreen Patrick LFujii Masahiro<p>Abstract</p> <p>Background</p> <p>The interaction of human T-cell leukemia virus type 1 (HTLV-1) Tax1 protein with the tumor suppressor Dlg1 is correlated with cellular transformation.</p> <p>Results</p> <p>Here, we show that Dlg1 knockdown by RNA interference increases the ability of Tax1 to transform a mouse T-cell line (CTLL-2), as measured interleukin (IL)-2-independent growth. A Tax1 mutant defective for the Dlg1 interaction showed reduced transformation of CTLL-2 compared to wild type Tax1, but the transformation was minimally affected by Dlg1 reduction. The few Tax1ΔC-transduced CTLL-2 cells that became transformed expressed less Dlg1 than parental cells, suggesting that Dlg1-low cells were selectively transformed by Tax1ΔC. Moreover, all human T-cell lines immortalized by HTLV-1, including the recombinant HTLV-1-containing Tax1ΔC, expressed less Dlg1 than control T-cell lines.</p> <p>Conclusion</p> <p>These results suggest that inactivation of Dlg1 augments Tax1-mediated transformation of CTLL-2, and PDZ protein(s) other than Dlg1 are critically involved in the transformation.</p> http://www.retrovirology.com/content/3/1/71 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Tanaka Yuetsu Oie Masayasu Yoshida Sakiko Takahashi Masahiko Higuchi Masaya Ishioka Kojiro Takahashi Sugata Xie Li Green Patrick L Fujii Masahiro |
spellingShingle |
Tanaka Yuetsu Oie Masayasu Yoshida Sakiko Takahashi Masahiko Higuchi Masaya Ishioka Kojiro Takahashi Sugata Xie Li Green Patrick L Fujii Masahiro Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein Retrovirology |
author_facet |
Tanaka Yuetsu Oie Masayasu Yoshida Sakiko Takahashi Masahiko Higuchi Masaya Ishioka Kojiro Takahashi Sugata Xie Li Green Patrick L Fujii Masahiro |
author_sort |
Tanaka Yuetsu |
title |
Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein |
title_short |
Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein |
title_full |
Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein |
title_fullStr |
Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein |
title_full_unstemmed |
Inactivation of tumor suppressor Dlg1 augments transformation of a T-cell line induced by human T-cell leukemia virus type 1 Tax protein |
title_sort |
inactivation of tumor suppressor dlg1 augments transformation of a t-cell line induced by human t-cell leukemia virus type 1 tax protein |
publisher |
BMC |
series |
Retrovirology |
issn |
1742-4690 |
publishDate |
2006-10-01 |
description |
<p>Abstract</p> <p>Background</p> <p>The interaction of human T-cell leukemia virus type 1 (HTLV-1) Tax1 protein with the tumor suppressor Dlg1 is correlated with cellular transformation.</p> <p>Results</p> <p>Here, we show that Dlg1 knockdown by RNA interference increases the ability of Tax1 to transform a mouse T-cell line (CTLL-2), as measured interleukin (IL)-2-independent growth. A Tax1 mutant defective for the Dlg1 interaction showed reduced transformation of CTLL-2 compared to wild type Tax1, but the transformation was minimally affected by Dlg1 reduction. The few Tax1ΔC-transduced CTLL-2 cells that became transformed expressed less Dlg1 than parental cells, suggesting that Dlg1-low cells were selectively transformed by Tax1ΔC. Moreover, all human T-cell lines immortalized by HTLV-1, including the recombinant HTLV-1-containing Tax1ΔC, expressed less Dlg1 than control T-cell lines.</p> <p>Conclusion</p> <p>These results suggest that inactivation of Dlg1 augments Tax1-mediated transformation of CTLL-2, and PDZ protein(s) other than Dlg1 are critically involved in the transformation.</p> |
url |
http://www.retrovirology.com/content/3/1/71 |
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