Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy
Infectious agents can reach the placenta either via the maternal blood or by ascending the genito-urinary tract, and then initially colonizing the maternal decidua. Decidual stromal cells (DSCs) are the major cellular component of the decidua. Although DSCs at the maternal–fetal interface contribute...
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Frontiers Media S.A.
2017-08-01
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doaj-d8a971d9ae8645e18262879c83c9eeb72020-11-24T21:15:39ZengFrontiers Media S.A.Frontiers in Immunology1664-32242017-08-01810.3389/fimmu.2017.00916276924Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine PregnancyShui-Xing Yu0Feng-Hua Zhou1Wei Chen2Gui-Mei Jiang3Chong-Tao Du4Gui-Qiu Hu5Zhen-Zhen Liu6Shi-Qing Yan7Jing-Min Gu8Xu-Ming Deng9Tong-Jun Lin10En-Kui Duan11Yong-Jun Yang12Key Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaDepartment of Microbiology and Immunology, Dalhousie University, Halifax, NS, CanadaState Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Zoonosis, Ministry of Education, College of Animal Medicine, Jilin University, Changchun, ChinaInfectious agents can reach the placenta either via the maternal blood or by ascending the genito-urinary tract, and then initially colonizing the maternal decidua. Decidual stromal cells (DSCs) are the major cellular component of the decidua. Although DSCs at the maternal–fetal interface contribute to the regulation of immunity in pregnancy in the face of immunological and physiological challenges, the roles of these DSCs during viral infection remain ill defined. Here, we characterized the response of DSCs to a synthetic double-stranded RNA molecule, polyinosinic-polycytidylic acid [poly(I:C)], which is a mimic of viral infection. We demonstrated that both transfection of cells with poly(I:C) and addition of extracellular (non-transfected) poly(I:C) trigger the necroptosis of DSCs and that this response is dependent on RIG-I-like receptor/IPS-1 signaling and the toll-like receptor 3/TIR-domain-containing adapter-inducing interferon-β pathway, respectively. Furthermore, following poly(I:C) challenge, pregnant mixed lineage kinase domain-like protein-deficient mice had fewer necrotic cells in the mesometrial decidual layer, as well as milder pathological changes in the uterine unit, than did wild-type mice. Collectively, our results establish that necroptosis is a contributing factor in poly(I:C)-triggered abnormal pregnancy and thereby indicate a novel therapeutic strategy for reducing the severity of the adverse effects of viral infections in pregnancy.http://journal.frontiersin.org/article/10.3389/fimmu.2017.00916/fullabortionmixed lineage kinase domain-like proteinnecroptosispregnancypolyinosinic-polycytidylic acid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shui-Xing Yu Feng-Hua Zhou Wei Chen Gui-Mei Jiang Chong-Tao Du Gui-Qiu Hu Zhen-Zhen Liu Shi-Qing Yan Jing-Min Gu Xu-Ming Deng Tong-Jun Lin En-Kui Duan Yong-Jun Yang |
spellingShingle |
Shui-Xing Yu Feng-Hua Zhou Wei Chen Gui-Mei Jiang Chong-Tao Du Gui-Qiu Hu Zhen-Zhen Liu Shi-Qing Yan Jing-Min Gu Xu-Ming Deng Tong-Jun Lin En-Kui Duan Yong-Jun Yang Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy Frontiers in Immunology abortion mixed lineage kinase domain-like protein necroptosis pregnancy polyinosinic-polycytidylic acid |
author_facet |
Shui-Xing Yu Feng-Hua Zhou Wei Chen Gui-Mei Jiang Chong-Tao Du Gui-Qiu Hu Zhen-Zhen Liu Shi-Qing Yan Jing-Min Gu Xu-Ming Deng Tong-Jun Lin En-Kui Duan Yong-Jun Yang |
author_sort |
Shui-Xing Yu |
title |
Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy |
title_short |
Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy |
title_full |
Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy |
title_fullStr |
Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy |
title_full_unstemmed |
Decidual Stromal Cell Necroptosis Contributes to Polyinosinic-Polycytidylic Acid-Triggered Abnormal Murine Pregnancy |
title_sort |
decidual stromal cell necroptosis contributes to polyinosinic-polycytidylic acid-triggered abnormal murine pregnancy |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2017-08-01 |
description |
Infectious agents can reach the placenta either via the maternal blood or by ascending the genito-urinary tract, and then initially colonizing the maternal decidua. Decidual stromal cells (DSCs) are the major cellular component of the decidua. Although DSCs at the maternal–fetal interface contribute to the regulation of immunity in pregnancy in the face of immunological and physiological challenges, the roles of these DSCs during viral infection remain ill defined. Here, we characterized the response of DSCs to a synthetic double-stranded RNA molecule, polyinosinic-polycytidylic acid [poly(I:C)], which is a mimic of viral infection. We demonstrated that both transfection of cells with poly(I:C) and addition of extracellular (non-transfected) poly(I:C) trigger the necroptosis of DSCs and that this response is dependent on RIG-I-like receptor/IPS-1 signaling and the toll-like receptor 3/TIR-domain-containing adapter-inducing interferon-β pathway, respectively. Furthermore, following poly(I:C) challenge, pregnant mixed lineage kinase domain-like protein-deficient mice had fewer necrotic cells in the mesometrial decidual layer, as well as milder pathological changes in the uterine unit, than did wild-type mice. Collectively, our results establish that necroptosis is a contributing factor in poly(I:C)-triggered abnormal pregnancy and thereby indicate a novel therapeutic strategy for reducing the severity of the adverse effects of viral infections in pregnancy. |
topic |
abortion mixed lineage kinase domain-like protein necroptosis pregnancy polyinosinic-polycytidylic acid |
url |
http://journal.frontiersin.org/article/10.3389/fimmu.2017.00916/full |
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