Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis
With the help of enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in 332 patients with acute feverish illness after a tick bite, treated at the Perm Regional Hospital of Infectious Diseases in the spring-summer period of 2010, the diagnostics of human granulocytic anapla...
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Journal Infectology
2014-09-01
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doaj-d8b5e37c2a6248f49f2a58202db106c42021-08-02T08:43:16ZrusJournal InfectologyŽurnal Infektologii 2072-67322014-09-0142333910.22625/2072-6732-2012-4-2-33-39110Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosisV. Yu. Teterin0E. I. Korenberg1V. V. Nefedova2N. N. Vorob’ova3V. I. Frizen4Perm State Medical Academy named by academician E.A. Wagner, PermResearch Institute of Epidemiology and Microbiology named by N.F. Gamaleya, MoscowResearch Institute of Epidemiology and Microbiology named by N.F. Gamaleya, MoscowPerm State Medical Academy named by academician E.A. Wagner, PermPerm Regional Hospital of Infectious Diseases, PermWith the help of enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in 332 patients with acute feverish illness after a tick bite, treated at the Perm Regional Hospital of Infectious Diseases in the spring-summer period of 2010, the diagnostics of human granulocytic anaplasmosis was performed. By Omnix’s testsystems HGA-ELISA-IgM and HGA-ELISA-IgG 845 serum, as well as by «nested» PCR, using primers ge3a1-ge10r2 and ge9f3-ge2r4 for A. phagocytophilum genetic material amplification, 952 samples of dried blood spots were studied in the dynamics of the disease. The optimal period for the use of ELISA and PCR was determined. Anaplasmosis was confirmed by both methods in 79 patients (ELISA – in 68, PCR –in 25). In general, serodiagnosis, particularly ELISA, and PCR should be regarded not as alternative but as complementary to each other’s methods of laboratory diagnosis of anaplasmosis.https://journal.niidi.ru/jofin/article/view/96human granulocytic anaplasmosisenzymelinked immunoassaypolymerase chain reaction |
collection |
DOAJ |
language |
Russian |
format |
Article |
sources |
DOAJ |
author |
V. Yu. Teterin E. I. Korenberg V. V. Nefedova N. N. Vorob’ova V. I. Frizen |
spellingShingle |
V. Yu. Teterin E. I. Korenberg V. V. Nefedova N. N. Vorob’ova V. I. Frizen Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis Žurnal Infektologii human granulocytic anaplasmosis enzymelinked immunoassay polymerase chain reaction |
author_facet |
V. Yu. Teterin E. I. Korenberg V. V. Nefedova N. N. Vorob’ova V. I. Frizen |
author_sort |
V. Yu. Teterin |
title |
Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis |
title_short |
Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis |
title_full |
Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis |
title_fullStr |
Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis |
title_full_unstemmed |
Enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis |
title_sort |
enzyme-linked immunosorbent assay and polymerase chain reaction in the laboratory diagnosis of human granulocytic anaplasmosis |
publisher |
Journal Infectology |
series |
Žurnal Infektologii |
issn |
2072-6732 |
publishDate |
2014-09-01 |
description |
With the help of enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) in 332 patients with acute feverish illness after a tick bite, treated at the Perm Regional Hospital of Infectious Diseases in the spring-summer period of 2010, the diagnostics of human granulocytic anaplasmosis was performed. By Omnix’s testsystems HGA-ELISA-IgM and HGA-ELISA-IgG 845 serum, as well as by «nested» PCR, using primers ge3a1-ge10r2 and ge9f3-ge2r4 for A. phagocytophilum genetic material amplification, 952 samples of dried blood spots were studied in the dynamics of the disease. The optimal period for the use of ELISA and PCR was determined. Anaplasmosis was confirmed by both methods in 79 patients (ELISA – in 68, PCR –in 25). In general, serodiagnosis, particularly ELISA, and PCR should be regarded not as alternative but as complementary to each other’s methods of laboratory diagnosis of anaplasmosis. |
topic |
human granulocytic anaplasmosis enzymelinked immunoassay polymerase chain reaction |
url |
https://journal.niidi.ru/jofin/article/view/96 |
work_keys_str_mv |
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