Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study
Uncalcined and unsintered hydroxyapatite/poly <span style="font-variant: small-caps;">l</span>-lactide (u-HA/PLLA) material has osteoconductive characteristics and is available for use as a maxillofacial osteosynthetic reconstruction device. However, its bone regeneration abili...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2019-09-01
|
Series: | Materials |
Subjects: | |
Online Access: | https://www.mdpi.com/1996-1944/12/18/2931 |
id |
doaj-d8c109826ac8406c9bdb9018a5337cdd |
---|---|
record_format |
Article |
spelling |
doaj-d8c109826ac8406c9bdb9018a5337cdd2020-11-24T21:59:50ZengMDPI AGMaterials1996-19442019-09-011218293110.3390/ma12182931ma12182931Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo StudyQuang Ngoc Dong0Takahiro Kanno1Yunpeng Bai2Jingjing Sha3Katsumi Hideshima4Department of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanDepartment of Oral and Maxillofacial Surgery, Shimane University Faculty of Medicine, Izumo, Shimane 693-8501, JapanUncalcined and unsintered hydroxyapatite/poly <span style="font-variant: small-caps;">l</span>-lactide (u-HA/PLLA) material has osteoconductive characteristics and is available for use as a maxillofacial osteosynthetic reconstruction device. However, its bone regeneration ability in the maxillofacial region has not been fully investigated. This study is the first to assess the bone regenerative potential of osteoconductive u-HA/PLLA material when it is used for repairing maxillofacial bone defects. A total of 21 Sprague-Dawley male rats were divided into three groups—the u-HA/PLLA, PLLA, or sham control groups. A critical size defect of 4 mm was created in the mandible of each rat. Then, the defect was covered with either a u-HA/PLLA or PLLA sheet on the buccal side. The rats in each group were sacrificed at 2, 4, or 8 weeks. The rats’ mandibles were sampled for histological analysis with hematoxylin and eosin staining, histomorphometry, and immunohistochemistry with Runx2 and osteocalcin (OCN) antibody. The amount of newly formed bone in the u-HA/PLLA group was significantly higher than that of the PLLA group. The expression of Runx2 and OCN in the u-HA/PLLA group was also significantly higher. These results demonstrate that the u-HA/PLLA material has excellent bone regenerative ability and confirm its applicability as a reconstructive device in maxillofacial surgery.https://www.mdpi.com/1996-1944/12/18/2931osteoconductivitybone defectbone regenerationmaxillofacial surgeryPLLAu-HA/PLLA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Quang Ngoc Dong Takahiro Kanno Yunpeng Bai Jingjing Sha Katsumi Hideshima |
spellingShingle |
Quang Ngoc Dong Takahiro Kanno Yunpeng Bai Jingjing Sha Katsumi Hideshima Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study Materials osteoconductivity bone defect bone regeneration maxillofacial surgery PLLA u-HA/PLLA |
author_facet |
Quang Ngoc Dong Takahiro Kanno Yunpeng Bai Jingjing Sha Katsumi Hideshima |
author_sort |
Quang Ngoc Dong |
title |
Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study |
title_short |
Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study |
title_full |
Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study |
title_fullStr |
Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study |
title_full_unstemmed |
Bone Regeneration Potential of Uncalcined and Unsintered Hydroxyapatite/Poly <span style="font-variant: small-caps">l</span>-lactide Bioactive/Osteoconductive Sheet Used for Maxillofacial Reconstructive Surgery: An In Vivo Study |
title_sort |
bone regeneration potential of uncalcined and unsintered hydroxyapatite/poly <span style="font-variant: small-caps">l</span>-lactide bioactive/osteoconductive sheet used for maxillofacial reconstructive surgery: an in vivo study |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2019-09-01 |
description |
Uncalcined and unsintered hydroxyapatite/poly <span style="font-variant: small-caps;">l</span>-lactide (u-HA/PLLA) material has osteoconductive characteristics and is available for use as a maxillofacial osteosynthetic reconstruction device. However, its bone regeneration ability in the maxillofacial region has not been fully investigated. This study is the first to assess the bone regenerative potential of osteoconductive u-HA/PLLA material when it is used for repairing maxillofacial bone defects. A total of 21 Sprague-Dawley male rats were divided into three groups—the u-HA/PLLA, PLLA, or sham control groups. A critical size defect of 4 mm was created in the mandible of each rat. Then, the defect was covered with either a u-HA/PLLA or PLLA sheet on the buccal side. The rats in each group were sacrificed at 2, 4, or 8 weeks. The rats’ mandibles were sampled for histological analysis with hematoxylin and eosin staining, histomorphometry, and immunohistochemistry with Runx2 and osteocalcin (OCN) antibody. The amount of newly formed bone in the u-HA/PLLA group was significantly higher than that of the PLLA group. The expression of Runx2 and OCN in the u-HA/PLLA group was also significantly higher. These results demonstrate that the u-HA/PLLA material has excellent bone regenerative ability and confirm its applicability as a reconstructive device in maxillofacial surgery. |
topic |
osteoconductivity bone defect bone regeneration maxillofacial surgery PLLA u-HA/PLLA |
url |
https://www.mdpi.com/1996-1944/12/18/2931 |
work_keys_str_mv |
AT quangngocdong boneregenerationpotentialofuncalcinedandunsinteredhydroxyapatitepolyspanstylefontvariantsmallcapslspanlactidebioactiveosteoconductivesheetusedformaxillofacialreconstructivesurgeryaninvivostudy AT takahirokanno boneregenerationpotentialofuncalcinedandunsinteredhydroxyapatitepolyspanstylefontvariantsmallcapslspanlactidebioactiveosteoconductivesheetusedformaxillofacialreconstructivesurgeryaninvivostudy AT yunpengbai boneregenerationpotentialofuncalcinedandunsinteredhydroxyapatitepolyspanstylefontvariantsmallcapslspanlactidebioactiveosteoconductivesheetusedformaxillofacialreconstructivesurgeryaninvivostudy AT jingjingsha boneregenerationpotentialofuncalcinedandunsinteredhydroxyapatitepolyspanstylefontvariantsmallcapslspanlactidebioactiveosteoconductivesheetusedformaxillofacialreconstructivesurgeryaninvivostudy AT katsumihideshima boneregenerationpotentialofuncalcinedandunsinteredhydroxyapatitepolyspanstylefontvariantsmallcapslspanlactidebioactiveosteoconductivesheetusedformaxillofacialreconstructivesurgeryaninvivostudy |
_version_ |
1725847073327153152 |