Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate
Middle East Respiratory Syndrome coronavirus (MERS-CoV) infects dromedary camels and zoonotically infects humans, causing a respiratory disease with severe pneumonia and death. With no approved antiviral or vaccine interventions for MERS, vaccines are being developed for camels to prevent virus tran...
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doaj-d8d3707c69ec4324bb68fa6f560ba8632021-08-26T14:26:13ZengMDPI AGVeterinary Sciences2306-73812021-08-01815615610.3390/vetsci8080156Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine CandidateSharif Hala0Paolo Ribeca1Haya A. Aljami2Suliman A. Alsagaby3Ibrahim Qasim4Sarah C. Gilbert5Naif Khalaf Alharbi6Vaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center (KAIMRC), Riyadh 11481, Saudi ArabiaBiomathematics and Statistics Scotland, The James Hutton Institute, Edinburgh EH9 3FD, UKVaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center (KAIMRC), Riyadh 11481, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah 11952, Saudi ArabiaMinistry of Environment, Water and Agriculture (MEWA), Riyadh 11481, Saudi ArabiaThe Jenner Institute, Nuffield Department of Medicine, University of Oxford, Oxford OX1 4BH, UKVaccine Development Unit, Department of Infectious Disease Research, King Abdullah International Medical Research Center (KAIMRC), Riyadh 11481, Saudi ArabiaMiddle East Respiratory Syndrome coronavirus (MERS-CoV) infects dromedary camels and zoonotically infects humans, causing a respiratory disease with severe pneumonia and death. With no approved antiviral or vaccine interventions for MERS, vaccines are being developed for camels to prevent virus transmission into humans. We have previously developed a chimpanzee adenoviral vector-based vaccine for MERS-CoV (ChAdOx1 MERS) and reported its strong humoral immunogenicity in dromedary camels. Here, we looked back at total RNA isolated from whole blood of three immunised dromedaries pre and post-vaccination during the first day; and performed RNA sequencing and bioinformatic analysis in order to shed light on the molecular immune responses following a ChAdOx1 MERS vaccination. Our finding shows that a number of transcripts were differentially regulated as an effect of the vaccination, including genes that are involved in innate and adaptive immunity, such as type I and II interferon responses. The camel Bcl-3 and Bcl-6 transcripts were significantly upregulated, indicating a strong activation of Tfh cell, B cell, and NF-κB pathways. In conclusion, this study gives an overall view of the first changes in the immune transcriptome of dromedaries after vaccination; it supports the potency of ChAdOx1 MERS as a potential camel vaccine to block transmission and prevent new human cases and outbreaks.https://www.mdpi.com/2306-7381/8/8/156transcriptomegene expressioncamelMERS-CoVvaccineimmunogenicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sharif Hala Paolo Ribeca Haya A. Aljami Suliman A. Alsagaby Ibrahim Qasim Sarah C. Gilbert Naif Khalaf Alharbi |
spellingShingle |
Sharif Hala Paolo Ribeca Haya A. Aljami Suliman A. Alsagaby Ibrahim Qasim Sarah C. Gilbert Naif Khalaf Alharbi Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate Veterinary Sciences transcriptome gene expression camel MERS-CoV vaccine immunogenicity |
author_facet |
Sharif Hala Paolo Ribeca Haya A. Aljami Suliman A. Alsagaby Ibrahim Qasim Sarah C. Gilbert Naif Khalaf Alharbi |
author_sort |
Sharif Hala |
title |
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate |
title_short |
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate |
title_full |
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate |
title_fullStr |
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate |
title_full_unstemmed |
Transcriptomic Profiling of Dromedary Camels Immunised with a MERS Vaccine Candidate |
title_sort |
transcriptomic profiling of dromedary camels immunised with a mers vaccine candidate |
publisher |
MDPI AG |
series |
Veterinary Sciences |
issn |
2306-7381 |
publishDate |
2021-08-01 |
description |
Middle East Respiratory Syndrome coronavirus (MERS-CoV) infects dromedary camels and zoonotically infects humans, causing a respiratory disease with severe pneumonia and death. With no approved antiviral or vaccine interventions for MERS, vaccines are being developed for camels to prevent virus transmission into humans. We have previously developed a chimpanzee adenoviral vector-based vaccine for MERS-CoV (ChAdOx1 MERS) and reported its strong humoral immunogenicity in dromedary camels. Here, we looked back at total RNA isolated from whole blood of three immunised dromedaries pre and post-vaccination during the first day; and performed RNA sequencing and bioinformatic analysis in order to shed light on the molecular immune responses following a ChAdOx1 MERS vaccination. Our finding shows that a number of transcripts were differentially regulated as an effect of the vaccination, including genes that are involved in innate and adaptive immunity, such as type I and II interferon responses. The camel Bcl-3 and Bcl-6 transcripts were significantly upregulated, indicating a strong activation of Tfh cell, B cell, and NF-κB pathways. In conclusion, this study gives an overall view of the first changes in the immune transcriptome of dromedaries after vaccination; it supports the potency of ChAdOx1 MERS as a potential camel vaccine to block transmission and prevent new human cases and outbreaks. |
topic |
transcriptome gene expression camel MERS-CoV vaccine immunogenicity |
url |
https://www.mdpi.com/2306-7381/8/8/156 |
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