Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin
Background: Parvalbumin is the major and cross-reactive allergen in fish. Sufficient amounts of IgE-reactive recombinant fish parvalbumin are needed for diagnosis and immunotherapy of fish allergy. Methods: A DNA fragment corresponding to parvalbumin of the Pacific mackerel Scomber japonicus was syn...
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doaj-d8dca85c9775454b93f4e2a1b79bd8b02020-11-24T22:43:48ZengElsevierAllergology International1323-89302004-01-0153327127810.1111/j.1440-1592.2004.00344.xExpression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbuminYuki Hamada0Hiroyuki Tanaka1Ayako Sato2Shoichiro Ishizaki3Yuji Nagashima4Kazuo Shiomi5Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, JapanDepartment of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo, JapanBackground: Parvalbumin is the major and cross-reactive allergen in fish. Sufficient amounts of IgE-reactive recombinant fish parvalbumin are needed for diagnosis and immunotherapy of fish allergy. Methods: A DNA fragment corresponding to parvalbumin of the Pacific mackerel Scomber japonicus was synthesized and cloned into the expression vector pGEX-6p-3 to produce glutathione S-transferase (GST)-fusion parvalbumin in Escherichia coli. The GST-free recombinant parvalbumin was purified using the RediPack GST Purification Module (Amersham Pharmacia Biotech, Buckinghamshire, UK). Parvalbumins of seven species of fish (Japanese eel, horse mackerel, red sea bream, Pacific mackerel, skipjack, bigeye tuna and Japanese flounder) were purified by gel filtration and reverse-phase HPLC. The IgE-binding capacity was examined by ELISA and antigenic cross-reactivity by inhibition ELISA. Results: The GST-free recombinant Pacific mackerel parvalbumin was obtained in an electrophoretically pure state. Data from ELISA and inhibition ELISA revealed that the recombinant parvalbumin contains most of the IgE-binding epitopes of the natural counterpart. In addition, the recombinant parvalbumin inhibited the IgE reactivities of the pooled patient serum to parvalbumins purified from six species of fish in almost the same magnitude as the natural Pacific mackerel parvalbumin. Conclusions: Because the recombinant Pacific mackerel parvalbumin bearing the IgE-binding capacity of the natural counterpart is cross-reactive with various fish parvalbumins, it can be a useful tool for the diagnosis and immunotherapy of fish allergy.http://www.sciencedirect.com/science/article/pii/S1323893015311278cross-reactivityexpressionIgE-binding capacitymackerelparvalbumin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuki Hamada Hiroyuki Tanaka Ayako Sato Shoichiro Ishizaki Yuji Nagashima Kazuo Shiomi |
spellingShingle |
Yuki Hamada Hiroyuki Tanaka Ayako Sato Shoichiro Ishizaki Yuji Nagashima Kazuo Shiomi Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin Allergology International cross-reactivity expression IgE-binding capacity mackerel parvalbumin |
author_facet |
Yuki Hamada Hiroyuki Tanaka Ayako Sato Shoichiro Ishizaki Yuji Nagashima Kazuo Shiomi |
author_sort |
Yuki Hamada |
title |
Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin |
title_short |
Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin |
title_full |
Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin |
title_fullStr |
Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin |
title_full_unstemmed |
Expression and evaluation of IgE-binding capacity of recombinant Pacific mackerel parvalbumin |
title_sort |
expression and evaluation of ige-binding capacity of recombinant pacific mackerel parvalbumin |
publisher |
Elsevier |
series |
Allergology International |
issn |
1323-8930 |
publishDate |
2004-01-01 |
description |
Background: Parvalbumin is the major and cross-reactive allergen in fish. Sufficient amounts of IgE-reactive recombinant fish parvalbumin are needed for diagnosis and immunotherapy of fish allergy.
Methods: A DNA fragment corresponding to parvalbumin of the Pacific mackerel Scomber japonicus was synthesized and cloned into the expression vector pGEX-6p-3 to produce glutathione S-transferase (GST)-fusion parvalbumin in Escherichia coli. The GST-free recombinant parvalbumin was purified using the RediPack GST Purification Module (Amersham Pharmacia Biotech, Buckinghamshire, UK). Parvalbumins of seven species of fish (Japanese eel, horse mackerel, red sea bream, Pacific mackerel, skipjack, bigeye tuna and Japanese flounder) were purified by gel filtration and reverse-phase HPLC. The IgE-binding capacity was examined by ELISA and antigenic cross-reactivity by inhibition ELISA.
Results: The GST-free recombinant Pacific mackerel parvalbumin was obtained in an electrophoretically pure state. Data from ELISA and inhibition ELISA revealed that the recombinant parvalbumin contains most of the IgE-binding epitopes of the natural counterpart. In addition, the recombinant parvalbumin inhibited the IgE reactivities of the pooled patient serum to parvalbumins purified from six species of fish in almost the same magnitude as the natural Pacific mackerel parvalbumin.
Conclusions: Because the recombinant Pacific mackerel parvalbumin bearing the IgE-binding capacity of the natural counterpart is cross-reactive with various fish parvalbumins, it can be a useful tool for the diagnosis and immunotherapy of fish allergy. |
topic |
cross-reactivity expression IgE-binding capacity mackerel parvalbumin |
url |
http://www.sciencedirect.com/science/article/pii/S1323893015311278 |
work_keys_str_mv |
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