DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)
A DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or ora...
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Centers for Disease Control and Prevention
2003-09-01
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Online Access: | https://wwwnc.cdc.gov/eid/article/9/9/03-0025_article |
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doaj-d8e0389e440f4868ba6bffd4aaea623d2020-11-25T01:35:12ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592003-09-01991077108110.3201/eid0909.030025DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus)Michael J. TurellMichel L. BunningGeorge V. LudwigBrian OrtmanJeff ChangTully SpeakerAndrew SpielmanRobert McLeanNicholas KomarRobert GatesTracey McNamaraTerry CreekmoreLinda FarleyCarl J. MitchellA DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or orally exposed to a placebo. After 6 weeks, crows were challenged subcutaneously with 105 plaque-forming units of WNV (New York 1999 strain). None of the placebo inoculated–placebo challenged birds died. While none of the 9 IM vaccine–inoculated birds died, 5 of 10 placebo-inoculated and 4 of 8 orally vaccinated birds died within 15 days after challenge. Peak viremia titers in birds with fatal WNV infection were substantially higher than those in birds that survived infection. Although oral administration of a single DNA vaccine dose failed to elicit an immune response or protect crows from WNV infection, IM administration of a single dose prevented death and was associated with reduced viremia.https://wwwnc.cdc.gov/eid/article/9/9/03-0025_articlevaccineWest Nile virusfish crowDNA vaccineflavivirusUnited States |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael J. Turell Michel L. Bunning George V. Ludwig Brian Ortman Jeff Chang Tully Speaker Andrew Spielman Robert McLean Nicholas Komar Robert Gates Tracey McNamara Terry Creekmore Linda Farley Carl J. Mitchell |
spellingShingle |
Michael J. Turell Michel L. Bunning George V. Ludwig Brian Ortman Jeff Chang Tully Speaker Andrew Spielman Robert McLean Nicholas Komar Robert Gates Tracey McNamara Terry Creekmore Linda Farley Carl J. Mitchell DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) Emerging Infectious Diseases vaccine West Nile virus fish crow DNA vaccine flavivirus United States |
author_facet |
Michael J. Turell Michel L. Bunning George V. Ludwig Brian Ortman Jeff Chang Tully Speaker Andrew Spielman Robert McLean Nicholas Komar Robert Gates Tracey McNamara Terry Creekmore Linda Farley Carl J. Mitchell |
author_sort |
Michael J. Turell |
title |
DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) |
title_short |
DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) |
title_full |
DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) |
title_fullStr |
DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) |
title_full_unstemmed |
DNA Vaccine for West Nile Virus Infection in Fish Crows (Corvus ossifragus) |
title_sort |
dna vaccine for west nile virus infection in fish crows (corvus ossifragus) |
publisher |
Centers for Disease Control and Prevention |
series |
Emerging Infectious Diseases |
issn |
1080-6040 1080-6059 |
publishDate |
2003-09-01 |
description |
A DNA vaccine for West Nile virus (WNV) was evaluated to determine whether its use could protect fish crows (Corvus ossifragus) from fatal WNV infection. Captured adult crows were given 0.5 mg of the DNA vaccine either orally or by intramuscular (IM) inoculation; control crows were inoculated or orally exposed to a placebo. After 6 weeks, crows were challenged subcutaneously with 105 plaque-forming units of WNV (New York 1999 strain). None of the placebo inoculated–placebo challenged birds died. While none of the 9 IM vaccine–inoculated birds died, 5 of 10 placebo-inoculated and 4 of 8 orally vaccinated birds died within 15 days after challenge. Peak viremia titers in birds with fatal WNV infection were substantially higher than those in birds that survived infection. Although oral administration of a single DNA vaccine dose failed to elicit an immune response or protect crows from WNV infection, IM administration of a single dose prevented death and was associated with reduced viremia. |
topic |
vaccine West Nile virus fish crow DNA vaccine flavivirus United States |
url |
https://wwwnc.cdc.gov/eid/article/9/9/03-0025_article |
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