Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration
Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β-glucuronidase (GUS...
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Universidad de Cordoba
2014-09-01
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doaj-d8f064eba2994a00af4b174212cb71462020-11-24T21:15:30ZengUniversidad de CordobaRevista MVZ Cordoba0122-02681909-05442014-09-0119343384349Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltrationPaula Díaz T.0Adriana Bernal G.1Camilo López C.2Universidad Nacional de Colombia, Science Faculty, Biology Department, Manihot Biotec Investigation Group, Calle 30 N 45-03, Bogota, Colombia.Universidad de los Andes, Science Faculty, Biology Department, LAMFU, Cra 1 N 18A- 12, Bogota, Colombia.Universidad Nacional de Colombia, Science Faculty, Biology Department, Manihot Biotec Investigation Group, Calle 30 N 45-03, Bogota, Colombia.Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β-glucuronidase (GUS). A. tumefaciens infiltration (agroinfiltration) was made using both leaves from in vitro and 1 month old greenhouse plants. Leaves were incubated in X-GLUC buffer, stained and photographed to detect GUS activity. Results. Agroinfiltration assays showed GUS transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, MCOL2215 (Venezuelan) and CM6438-14 (Vergara), respectively. A. tumefaciens agressive strain AGL1 showed high efficiency inducing GUS expression in cassava leaves. Conclusions. We recommend using A. tumefaciens agressive strain AGL1 for agroinfiltration to assess transient expression in cassava leaves.http://revistas.unicordoba.edu.co/revistamvz/mvz-193/v19n3a14.pdfCassavatransient gene expressionAgrobacterium tumefaciensbeta-glucuronidase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Paula Díaz T. Adriana Bernal G. Camilo López C. |
spellingShingle |
Paula Díaz T. Adriana Bernal G. Camilo López C. Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration Revista MVZ Cordoba Cassava transient gene expression Agrobacterium tumefaciens beta-glucuronidase |
author_facet |
Paula Díaz T. Adriana Bernal G. Camilo López C. |
author_sort |
Paula Díaz T. |
title |
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration |
title_short |
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration |
title_full |
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration |
title_fullStr |
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration |
title_full_unstemmed |
Transient GUS gene expression in cassava (Manihot esculenta Crantz) using Agrobacterium tumefaciens leaf infiltration |
title_sort |
transient gus gene expression in cassava (manihot esculenta crantz) using agrobacterium tumefaciens leaf infiltration |
publisher |
Universidad de Cordoba |
series |
Revista MVZ Cordoba |
issn |
0122-0268 1909-0544 |
publishDate |
2014-09-01 |
description |
Objective. Assess transient gene expression of GUS in cassava (Manihot esculenta Crantz) leaves using Agrobacterium tumefaciens infiltration. Materials and methods. A. tumefaciens strains GV3101 and AGL1 containing pCAMBIA1305.2 were used to evaluate transient gene expression of β-glucuronidase (GUS). A. tumefaciens infiltration (agroinfiltration) was made using both leaves from in vitro and 1 month old greenhouse plants. Leaves were incubated in X-GLUC buffer, stained and photographed to detect GUS activity. Results. Agroinfiltration assays showed GUS transient expression in leaves of cassava varieties widely cultivated in the north coast and eastern savannah, MCOL2215 (Venezuelan) and CM6438-14 (Vergara), respectively. A. tumefaciens agressive strain AGL1 showed high efficiency inducing GUS expression in cassava leaves. Conclusions. We recommend using A. tumefaciens agressive strain AGL1 for agroinfiltration to assess transient expression in cassava leaves. |
topic |
Cassava transient gene expression Agrobacterium tumefaciens beta-glucuronidase |
url |
http://revistas.unicordoba.edu.co/revistamvz/mvz-193/v19n3a14.pdf |
work_keys_str_mv |
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