HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING
This paper presents the results of experimental and theoretical studies of quenching and enhancement of fluorescence by colloidal solutions of nanoparticles and arrays of nanostructures on solid substrates — nanochips. The literature data and the results of authors’ own studies on the possibility of...
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National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.
2015-10-01
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doaj-d8f141578bf84c6388c16a09a2b70ec12020-11-24T23:34:37ZengNational Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine.Biotechnologia Acta2410-77512410-776X2015-10-018591810.15407/biotech8.05.009HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCINGV. I. Chegel0A. M. Lopatynskyi1V. К. Lytvyn2O. M. Naum3А. P. Demchenkо4V. I. Nazarenko5Lashkaryov Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, KyivLashkaryov Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, KyivLashkaryov Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, KyivLashkaryov Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivPalladin Institute of Biochemistry of the National Academy of Sciences of Ukraine, KyivThis paper presents the results of experimental and theoretical studies of quenching and enhancement of fluorescence by colloidal solutions of nanoparticles and arrays of nanostructures on solid substrates — nanochips. The literature data and the results of authors’ own studies on the possibility of fluorescence signal manipulation in the presence of gold and silver nanostructures were shown. Mathematical modeling and comparative investigation of the samples with high-conductive metal nanostructures as active elements for the regulation of fluorescence signal were also performed. Nanochips samples were fabricated by thermal annealing of highly conductive gold and silver island films. Using developed novel laser-based fluorometer FluorotestNano it was shown that fluorescence intensity of Rhodamine 6G dye can be enhanced up to 23 times near gold nanostructures by spacing the dye from the nanoparticle at the distance of 20 nm using SiO2 coating. Using high-conductive metal nanostructures to adjust the fluorescence signal opens promising new directions in biochemical studies, such as increasing the sensitivity of fluorescence methods, development of new biosensors, fluorescence microscopy techniques and medical diagnostics.http://biotechnology.kiev.ua/images/stories/5_2015/chegel.pdfnanostructure arraysgold and silver nanoparticlessurface-enhanced fluorescencepolymer matrix |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
V. I. Chegel A. M. Lopatynskyi V. К. Lytvyn O. M. Naum А. P. Demchenkо V. I. Nazarenko |
spellingShingle |
V. I. Chegel A. M. Lopatynskyi V. К. Lytvyn O. M. Naum А. P. Demchenkо V. I. Nazarenko HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING Biotechnologia Acta nanostructure arrays gold and silver nanoparticles surface-enhanced fluorescence polymer matrix |
author_facet |
V. I. Chegel A. M. Lopatynskyi V. К. Lytvyn O. M. Naum А. P. Demchenkо V. I. Nazarenko |
author_sort |
V. I. Chegel |
title |
HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING |
title_short |
HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING |
title_full |
HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING |
title_fullStr |
HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING |
title_full_unstemmed |
HIGH-CONDUCTIVE NANOSTRUCTURES IN BIOCHEMICAL STUDIES: FLUORESCENCE ENHANCING |
title_sort |
high-conductive nanostructures in biochemical studies: fluorescence enhancing |
publisher |
National Academy of Sciences of Ukraine and Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine. |
series |
Biotechnologia Acta |
issn |
2410-7751 2410-776X |
publishDate |
2015-10-01 |
description |
This paper presents the results of experimental and theoretical studies of quenching and enhancement of fluorescence by colloidal solutions of nanoparticles and arrays of nanostructures on solid substrates — nanochips. The literature data and the results of authors’ own studies on the possibility of fluorescence signal manipulation in the presence of gold and silver nanostructures were shown. Mathematical modeling and comparative investigation of the samples with high-conductive metal nanostructures as active elements for the regulation of fluorescence signal were also performed. Nanochips samples were fabricated by thermal annealing of highly conductive gold and silver island films. Using developed novel laser-based fluorometer FluorotestNano it was shown that fluorescence intensity of Rhodamine 6G dye can be enhanced up to 23 times near gold nanostructures by spacing the dye from the nanoparticle at the distance of 20 nm using SiO2 coating. Using high-conductive metal nanostructures to adjust the fluorescence signal opens promising new directions in biochemical studies, such as increasing the sensitivity of fluorescence methods, development of new biosensors, fluorescence microscopy techniques and medical diagnostics. |
topic |
nanostructure arrays gold and silver nanoparticles surface-enhanced fluorescence polymer matrix |
url |
http://biotechnology.kiev.ua/images/stories/5_2015/chegel.pdf |
work_keys_str_mv |
AT vichegel highconductivenanostructuresinbiochemicalstudiesfluorescenceenhancing AT amlopatynskyi highconductivenanostructuresinbiochemicalstudiesfluorescenceenhancing AT vklytvyn highconductivenanostructuresinbiochemicalstudiesfluorescenceenhancing AT omnaum highconductivenanostructuresinbiochemicalstudiesfluorescenceenhancing AT apdemchenko highconductivenanostructuresinbiochemicalstudiesfluorescenceenhancing AT vinazarenko highconductivenanostructuresinbiochemicalstudiesfluorescenceenhancing |
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1725528395819778048 |