Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus
The effects of status epilepticus on the orexin/hypocretin system have yet to be investigated. The present study aimed to assay orexin-A/hypocretin-1 in the cerebrospinal fluid (CSF) of patients after generalized convulsive status epilepticus (GCSE). The study groups included 20 GCSE patients, 24 pa...
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doaj-d9120454031e4bca8834d0e31fe3a7072020-11-25T04:00:57ZengMDPI AGJournal of Clinical Medicine2077-03832020-10-0193354335410.3390/jcm9103354Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status EpilepticusMojdeh Samzadeh0Ewa Papuć1Marzena Furtak-Niczyporuk2Konrad Rejdak3Department of Neurology, Medical University of Lublin, 20-954 Lublin, PolandDepartment of Neurology, Medical University of Lublin, 20-954 Lublin, PolandDepartment of Public Health, Medical University of Lublin, 20-954 Lublin, PolandDepartment of Neurology, Medical University of Lublin, 20-954 Lublin, PolandThe effects of status epilepticus on the orexin/hypocretin system have yet to be investigated. The present study aimed to assay orexin-A/hypocretin-1 in the cerebrospinal fluid (CSF) of patients after generalized convulsive status epilepticus (GCSE). The study groups included 20 GCSE patients, 24 patients diagnosed with epilepsy but remaining in remission (ER), and 25 normal controls (CTR). Diagnostic lumbar puncture was performed in GCSE patients within 3–10 days of seizure cessation, as well as in the ER and to CTR subjects. Among all GCSE patients, the outcome was graded according to the modified Rankin Scale (mRS) at 1-month follow-up. Orexin-A levels were measured in unextracted CSF samples, using a commercial radioimmunoassay. There was a significant overall difference in median CSF orexin-A concentrations between GCSE, RE, and CTR patients (<i>p </i><<i> </i>0.001). The lowest concentrations were noted in the GCSE group compared to ER (<i>p </i><<i> </i>0.001) or CTR (<i>p </i><<i> </i>0.001). CSF orexin-A levels in GCSE patients inversely correlated with clinical outcome as assessed on the mRS at 1-month follow-up (<i>r</i> = −0.55; <i>p </i>=<i> </i>0.1). In conclusion, CSF orexin-A levels may serve as a biomarker of increased turn-over of the peptide or post-SE neuronal damage, and implicates the orexin system in the pathogenesis of SE.https://www.mdpi.com/2077-0383/9/10/3354biomarkergeneralized convulsive status epilepticushypocretin 1orexin-Acerebrospinal fluid |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mojdeh Samzadeh Ewa Papuć Marzena Furtak-Niczyporuk Konrad Rejdak |
spellingShingle |
Mojdeh Samzadeh Ewa Papuć Marzena Furtak-Niczyporuk Konrad Rejdak Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus Journal of Clinical Medicine biomarker generalized convulsive status epilepticus hypocretin 1 orexin-A cerebrospinal fluid |
author_facet |
Mojdeh Samzadeh Ewa Papuć Marzena Furtak-Niczyporuk Konrad Rejdak |
author_sort |
Mojdeh Samzadeh |
title |
Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus |
title_short |
Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus |
title_full |
Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus |
title_fullStr |
Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus |
title_full_unstemmed |
Decreased Cerebrospinal Fluid Orexin-A (Hypocretin-1) Concentrations in Patients after Generalized Convulsive Status Epilepticus |
title_sort |
decreased cerebrospinal fluid orexin-a (hypocretin-1) concentrations in patients after generalized convulsive status epilepticus |
publisher |
MDPI AG |
series |
Journal of Clinical Medicine |
issn |
2077-0383 |
publishDate |
2020-10-01 |
description |
The effects of status epilepticus on the orexin/hypocretin system have yet to be investigated. The present study aimed to assay orexin-A/hypocretin-1 in the cerebrospinal fluid (CSF) of patients after generalized convulsive status epilepticus (GCSE). The study groups included 20 GCSE patients, 24 patients diagnosed with epilepsy but remaining in remission (ER), and 25 normal controls (CTR). Diagnostic lumbar puncture was performed in GCSE patients within 3–10 days of seizure cessation, as well as in the ER and to CTR subjects. Among all GCSE patients, the outcome was graded according to the modified Rankin Scale (mRS) at 1-month follow-up. Orexin-A levels were measured in unextracted CSF samples, using a commercial radioimmunoassay. There was a significant overall difference in median CSF orexin-A concentrations between GCSE, RE, and CTR patients (<i>p </i><<i> </i>0.001). The lowest concentrations were noted in the GCSE group compared to ER (<i>p </i><<i> </i>0.001) or CTR (<i>p </i><<i> </i>0.001). CSF orexin-A levels in GCSE patients inversely correlated with clinical outcome as assessed on the mRS at 1-month follow-up (<i>r</i> = −0.55; <i>p </i>=<i> </i>0.1). In conclusion, CSF orexin-A levels may serve as a biomarker of increased turn-over of the peptide or post-SE neuronal damage, and implicates the orexin system in the pathogenesis of SE. |
topic |
biomarker generalized convulsive status epilepticus hypocretin 1 orexin-A cerebrospinal fluid |
url |
https://www.mdpi.com/2077-0383/9/10/3354 |
work_keys_str_mv |
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