Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i>
The whitefly <i>Bemisia tabaci</i> is a globally important pest that is difficult to control through insecticides, transgenic crops, and natural enemies. Post-transcriptional gene silencing through RNA interference (RNAi) has shown potential as a pest management strategy against <i>...
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doaj-d9287cbb8d2a497f9a92443fb78838592020-11-25T03:57:44ZengMDPI AGInsects2075-44502020-10-011172372310.3390/insects11110723Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i>Emily A. Shelby0Jeanette B. Moss1Sharon A. Andreason2Alvin M. Simmons3Allen J. Moore4Patricia J. Moore5Department of Entomology, University of Georgia, Athens, GA 30602, USADepartment of Entomology, University of Georgia, Athens, GA 30602, USAU.S. Department of Agriculture, Agricultural Research Service, U.S. Vegetable laboratory, Charleston, SC 29414, USAU.S. Department of Agriculture, Agricultural Research Service, U.S. Vegetable laboratory, Charleston, SC 29414, USADepartment of Entomology, University of Georgia, Athens, GA 30602, USADepartment of Entomology, University of Georgia, Athens, GA 30602, USAThe whitefly <i>Bemisia tabaci</i> is a globally important pest that is difficult to control through insecticides, transgenic crops, and natural enemies. Post-transcriptional gene silencing through RNA interference (RNAi) has shown potential as a pest management strategy against <i>B. tabaci</i>. While genomic data and other resources are available to create highly effective customizable pest management strategies with RNAi, current applications do not capitalize on species-specific biology. This lack of specificity has the potential to have substantial ecological impacts. Here, we discuss both short- and long-term considerations for sustainable RNAi pest management strategies for <i>B. tabaci</i>, focusing on the need for species specificity incorporating both life history and population genetic considerations. We provide a conceptual framework for selecting sublethal target genes based on their involvement in physiological pathways, which has the greatest potential to ameliorate unintended negative consequences. We suggest that these considerations allow an integrated pest management approach, with fewer negative ecological impacts and reduced likelihood of the evolution of resistant populations.https://www.mdpi.com/2075-4450/11/11/723whitefly<i>Bemisia tabaci</i>RNAiRNA interferenceintegrated pest management |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Emily A. Shelby Jeanette B. Moss Sharon A. Andreason Alvin M. Simmons Allen J. Moore Patricia J. Moore |
spellingShingle |
Emily A. Shelby Jeanette B. Moss Sharon A. Andreason Alvin M. Simmons Allen J. Moore Patricia J. Moore Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i> Insects whitefly <i>Bemisia tabaci</i> RNAi RNA interference integrated pest management |
author_facet |
Emily A. Shelby Jeanette B. Moss Sharon A. Andreason Alvin M. Simmons Allen J. Moore Patricia J. Moore |
author_sort |
Emily A. Shelby |
title |
Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i> |
title_short |
Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i> |
title_full |
Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i> |
title_fullStr |
Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i> |
title_full_unstemmed |
Debugging: Strategies and Considerations for Efficient RNAi-Mediated Control of the Whitefly <i>Bemisia tabaci</i> |
title_sort |
debugging: strategies and considerations for efficient rnai-mediated control of the whitefly <i>bemisia tabaci</i> |
publisher |
MDPI AG |
series |
Insects |
issn |
2075-4450 |
publishDate |
2020-10-01 |
description |
The whitefly <i>Bemisia tabaci</i> is a globally important pest that is difficult to control through insecticides, transgenic crops, and natural enemies. Post-transcriptional gene silencing through RNA interference (RNAi) has shown potential as a pest management strategy against <i>B. tabaci</i>. While genomic data and other resources are available to create highly effective customizable pest management strategies with RNAi, current applications do not capitalize on species-specific biology. This lack of specificity has the potential to have substantial ecological impacts. Here, we discuss both short- and long-term considerations for sustainable RNAi pest management strategies for <i>B. tabaci</i>, focusing on the need for species specificity incorporating both life history and population genetic considerations. We provide a conceptual framework for selecting sublethal target genes based on their involvement in physiological pathways, which has the greatest potential to ameliorate unintended negative consequences. We suggest that these considerations allow an integrated pest management approach, with fewer negative ecological impacts and reduced likelihood of the evolution of resistant populations. |
topic |
whitefly <i>Bemisia tabaci</i> RNAi RNA interference integrated pest management |
url |
https://www.mdpi.com/2075-4450/11/11/723 |
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