New PHA products using unrelated carbon sources
Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia spe...
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Sociedade Brasileira de Microbiologia
2011-12-01
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doaj-d943ed715a1244479ebc3682dc5e3abf2020-11-24T22:22:29ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052011-12-014241354136310.1590/S1517-83822011000400017New PHA products using unrelated carbon sourcesFernanda MatiasMaria Filomena de Andrade RodriguesPolyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type, Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources. The classification was based on 16S rDNA and recA partial sequence genes and cell wall fatty acids composition. These two strains were capable to produce different types of PHA monomers or precursors. Unrelated carbon sources were used for growth and PHA accumulation. The amount of carbon source evaluated, or mixtures of them, was increased with every new experiment until it reaches eighteen carbon sources. As first bioprospection experiments staining methods were used with colony fluorescent dye Nile Red and the cell fluorescent dye Nile Blue A. Gas chromatography analysis coupled to mass spectrometry was used to evaluate the PHA composition on each strain cultivated on different carbon sources. The synthesized polymers were composed by short chain length-PHA (scl-PHA), especially polyhydroxybutyrate, and medium chain length-PHA (mcl-PHA) depending on the carbon source used.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400017BurkholderiaBurkholderia cepacia complexGenomovar-typemcl-PHApolyhydroxyalkanoatespolyhydroxybutyrate |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fernanda Matias Maria Filomena de Andrade Rodrigues |
spellingShingle |
Fernanda Matias Maria Filomena de Andrade Rodrigues New PHA products using unrelated carbon sources Brazilian Journal of Microbiology Burkholderia Burkholderia cepacia complex Genomovar-type mcl-PHA polyhydroxyalkanoates polyhydroxybutyrate |
author_facet |
Fernanda Matias Maria Filomena de Andrade Rodrigues |
author_sort |
Fernanda Matias |
title |
New PHA products using unrelated carbon sources |
title_short |
New PHA products using unrelated carbon sources |
title_full |
New PHA products using unrelated carbon sources |
title_fullStr |
New PHA products using unrelated carbon sources |
title_full_unstemmed |
New PHA products using unrelated carbon sources |
title_sort |
new pha products using unrelated carbon sources |
publisher |
Sociedade Brasileira de Microbiologia |
series |
Brazilian Journal of Microbiology |
issn |
1517-8382 1678-4405 |
publishDate |
2011-12-01 |
description |
Polyhydroxyalkanoates (PHA) are natural polyesters stored by a wide range of bacteria as carbon source reserve. Due to its chemical characteristics and biodegradability PHA can be used in chemical, medical and pharmaceutical industry for many human purposes. Over the past years, few Burkholderia species have become known for production of PHA. Aside from that, these bacteria seem to be interesting for discovering new PHA compositions which is important to different industrial applications. In this paper, we introduce two new strains which belong either to Burkholderia cepacia complex (Bcc) or genomovar-type, Burkholderia cepacia SA3J and Burkholderia contaminans I29B, both PHA producers from unrelated carbon sources. The classification was based on 16S rDNA and recA partial sequence genes and cell wall fatty acids composition. These two strains were capable to produce different types of PHA monomers or precursors. Unrelated carbon sources were used for growth and PHA accumulation. The amount of carbon source evaluated, or mixtures of them, was increased with every new experiment until it reaches eighteen carbon sources. As first bioprospection experiments staining methods were used with colony fluorescent dye Nile Red and the cell fluorescent dye Nile Blue A. Gas chromatography analysis coupled to mass spectrometry was used to evaluate the PHA composition on each strain cultivated on different carbon sources. The synthesized polymers were composed by short chain length-PHA (scl-PHA), especially polyhydroxybutyrate, and medium chain length-PHA (mcl-PHA) depending on the carbon source used. |
topic |
Burkholderia Burkholderia cepacia complex Genomovar-type mcl-PHA polyhydroxyalkanoates polyhydroxybutyrate |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400017 |
work_keys_str_mv |
AT fernandamatias newphaproductsusingunrelatedcarbonsources AT mariafilomenadeandraderodrigues newphaproductsusingunrelatedcarbonsources |
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