Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation
Biomaterial scaffolds play crucial role to promote cell proliferation and foster the regeneration of new tissues. The progress in material science has paved the way for the generation of ingenious biomaterials. However, these biomaterials require further optimization to be effectively used in existi...
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doaj-d94a764c76f746c2ac48dbff9d9d6a1f2020-11-25T04:04:42ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.567693567693Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell ProliferationSevakumaran Vigneswari0Jun Meng Chai1Khadijah Hilmun Kamarudin2Al-Ashraf Abdullah Amirul3Al-Ashraf Abdullah Amirul4Maria Letizia Focarete5Maria Letizia Focarete6Seeram Ramakrishna7Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Terengganu, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Terengganu, MalaysiaFaculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Terengganu, MalaysiaSchool of Biological Sciences, Universiti Sains Malaysia, George Town, MalaysiaCentre for Chemical Biology, Universiti Sains Malaysia, Bayan Lepas, MalaysiaDepartment of Chemistry “Giacomo Ciamician” and INSTM UdR of Bologna, University of Bologna, Bologna, ItalyHealth Sciences and Technologies-Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, Ozzano Emilia, ItalyDepartment of Mechanical Engineering, Center for Nanofibers and Nanotechnology, National University of Singapore, Singapore, SingaporeBiomaterial scaffolds play crucial role to promote cell proliferation and foster the regeneration of new tissues. The progress in material science has paved the way for the generation of ingenious biomaterials. However, these biomaterials require further optimization to be effectively used in existing clinical treatments. It is crucial to develop biomaterials which mimics structure that can be actively involved in delivering signals to cells for the formation of the regenerated tissue. In this research we nanoengineered a functional scaffold to support the proliferation of myoblast cells. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is chosen as scaffold material owing to its desirable mechanical and physical properties combined with good biocompatibility, thus eliciting appropriate host tissue responses. In this study P(3HB-co-4HB) copolymer was biosynthesized using Cupriavidus malaysiensis USMAA1020 transformant harboring additional PHA synthase gene, and the viability of a novel P(3HB-co-4HB) electrospun nanofiber scaffold, surface functionalized with RGD peptides, was explored. In order to immobilize RGD peptides molecules onto the P(3HB-co-4HB) nanofibers surface, an aminolysis reaction was performed. The nanoengineered scaffolds were characterized using SEM, organic elemental analysis (CHN analysis), FTIR, surface wettability and their in vitro degradation behavior was evaluated. The cell culture study using H9c2 myoblast cells was conducted to assess the in vitro cellular response of the engineered scaffold. Our results demonstrated that nano-P(3HB-co-4HB)-RGD scaffold possessed an average fiber diameter distribution between 200 and 300 nm, closely biomimicking, from a morphological point of view, the structural ECM components, thus acting as potential ECM analogs. This study indicates that the surface conjugation of biomimetic RGD peptide to the nano-P(3HB-co-4HB) fibers increased the surface wettability (15 ± 2°) and enhanced H9c2 myoblast cells attachment and proliferation. In summary, the study reveals that nano-P(3HB-co-4HB)-RGD scaffold can be considered a promising candidate to be further explored as cardiac construct for building cardiac construct.https://www.frontiersin.org/articles/10.3389/fbioe.2020.567693/fullP(3HB-co-4HB) nanofibersRGD peptidesaminolysismyoblast cellselectrospinning |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sevakumaran Vigneswari Jun Meng Chai Khadijah Hilmun Kamarudin Al-Ashraf Abdullah Amirul Al-Ashraf Abdullah Amirul Maria Letizia Focarete Maria Letizia Focarete Seeram Ramakrishna |
spellingShingle |
Sevakumaran Vigneswari Jun Meng Chai Khadijah Hilmun Kamarudin Al-Ashraf Abdullah Amirul Al-Ashraf Abdullah Amirul Maria Letizia Focarete Maria Letizia Focarete Seeram Ramakrishna Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation Frontiers in Bioengineering and Biotechnology P(3HB-co-4HB) nanofibers RGD peptides aminolysis myoblast cells electrospinning |
author_facet |
Sevakumaran Vigneswari Jun Meng Chai Khadijah Hilmun Kamarudin Al-Ashraf Abdullah Amirul Al-Ashraf Abdullah Amirul Maria Letizia Focarete Maria Letizia Focarete Seeram Ramakrishna |
author_sort |
Sevakumaran Vigneswari |
title |
Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation |
title_short |
Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation |
title_full |
Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation |
title_fullStr |
Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation |
title_full_unstemmed |
Elucidating the Surface Functionality of Biomimetic RGD Peptides Immobilized on Nano-P(3HB-co-4HB) for H9c2 Myoblast Cell Proliferation |
title_sort |
elucidating the surface functionality of biomimetic rgd peptides immobilized on nano-p(3hb-co-4hb) for h9c2 myoblast cell proliferation |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Bioengineering and Biotechnology |
issn |
2296-4185 |
publishDate |
2020-10-01 |
description |
Biomaterial scaffolds play crucial role to promote cell proliferation and foster the regeneration of new tissues. The progress in material science has paved the way for the generation of ingenious biomaterials. However, these biomaterials require further optimization to be effectively used in existing clinical treatments. It is crucial to develop biomaterials which mimics structure that can be actively involved in delivering signals to cells for the formation of the regenerated tissue. In this research we nanoengineered a functional scaffold to support the proliferation of myoblast cells. Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] copolymer is chosen as scaffold material owing to its desirable mechanical and physical properties combined with good biocompatibility, thus eliciting appropriate host tissue responses. In this study P(3HB-co-4HB) copolymer was biosynthesized using Cupriavidus malaysiensis USMAA1020 transformant harboring additional PHA synthase gene, and the viability of a novel P(3HB-co-4HB) electrospun nanofiber scaffold, surface functionalized with RGD peptides, was explored. In order to immobilize RGD peptides molecules onto the P(3HB-co-4HB) nanofibers surface, an aminolysis reaction was performed. The nanoengineered scaffolds were characterized using SEM, organic elemental analysis (CHN analysis), FTIR, surface wettability and their in vitro degradation behavior was evaluated. The cell culture study using H9c2 myoblast cells was conducted to assess the in vitro cellular response of the engineered scaffold. Our results demonstrated that nano-P(3HB-co-4HB)-RGD scaffold possessed an average fiber diameter distribution between 200 and 300 nm, closely biomimicking, from a morphological point of view, the structural ECM components, thus acting as potential ECM analogs. This study indicates that the surface conjugation of biomimetic RGD peptide to the nano-P(3HB-co-4HB) fibers increased the surface wettability (15 ± 2°) and enhanced H9c2 myoblast cells attachment and proliferation. In summary, the study reveals that nano-P(3HB-co-4HB)-RGD scaffold can be considered a promising candidate to be further explored as cardiac construct for building cardiac construct. |
topic |
P(3HB-co-4HB) nanofibers RGD peptides aminolysis myoblast cells electrospinning |
url |
https://www.frontiersin.org/articles/10.3389/fbioe.2020.567693/full |
work_keys_str_mv |
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