BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT
Andrographis paniculata or sambiloto is one of the most widely used medicinal herbs in Indonesia. The main bioactive chemical constituent, andrographolide, has been reported to have various pharmacological activities. Besides its function for medical purposes, the sambiloto herbs infusion is fre...
Main Authors: | , , , , , , , , , |
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Format: | Article |
Language: | English |
Published: |
Universitas Gadjah Mada
2014-07-01
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Series: | Indonesian Journal of Pharmacy |
Subjects: | |
Online Access: | http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/215 |
Summary: | Andrographis paniculata or sambiloto is one of the most
widely used medicinal herbs in Indonesia. The main bioactive
chemical constituent, andrographolide, has been reported to have
various pharmacological activities. Besides its function for medical
purposes, the sambiloto herbs infusion is frequently taken to
maintain health. This study was conducted to determine the
bioavailability of sambiloto herbs infusion in rabbit plasma,
stomach, and liver, calculated as total andrographolide. Fourteen
male New Zealand white rabbits were used in this study. Sambiloto
herbs infusion were administered orally at the dose 7.04mL/kg
body weight to each rabbit. Blood samples were taken at intervals
0.0; 0.5; 1.5; 2.0; 3.0; and 5.0h after infusion administration.
Sambiloto herbs infusion, which are calculated as andrographolide,
levels in plasma, stomach, and liver were analyzed by high
performance liquid chromatography using C-18 column as stationary
phase and a mixture of methanol-double distilled water (60:40) as
mobile phase. Bioavailability parameters obtained were Cmax
0.5549µg/mL (in stomach), 0.2136µg/mL (in plasma), 0.0051µg/mL
(in liver); while tmax 1h (in stomach), 1.5h (in plasma), 2h (in
liver); and AUC 1.7451µg.h/mL (in stomach), 0.434µg.h/mL (in
plasma), 0.0038µg.h/mL (in liver). These data showed that in
healthy animals, sambiloto herbs infusion was fastly absorbed
from the stomach, distributed in the circulation system, and
metabolized in the liver, in subsequent process. Sambiloto herbs
infusion showed good bioavailability in rabbit. |
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ISSN: | 2338-9427 2338-9486 |