BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT
Andrographis paniculata or sambiloto is one of the most widely used medicinal herbs in Indonesia. The main bioactive chemical constituent, andrographolide, has been reported to have various pharmacological activities. Besides its function for medical purposes, the sambiloto herbs infusion is fre...
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doaj-d958df9ba3c64aeab4138dd507c6710c2020-11-25T00:44:39ZengUniversitas Gadjah MadaIndonesian Journal of Pharmacy2338-94272338-94862014-07-0125313814410.14499/indonesianjpharm25iss3pp138BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBITJutti Levita0Hetsa Himawati1, Rizky Vitriani Lukman2Melysa Afdila3 Holis Abdul Holik4Nyi Mekar Saptarini5Aliya Nur Hasanah6Aliya Nur Hasanah7 As’ari Nawawi8Slamet Ibrahim9Abdul Mutalib10Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Dept. of Pharmaceutical Analysis and Medicinal Chemistry Faculty of Pharmacy Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363School of Pharmacy Institut Teknologi Bandung Jl. Ganesha 10 Bandung West Java IndonesiaSchool of Pharmacy Institut Teknologi Bandung Jl. Ganesha 10 Bandung West Java IndonesiaDept of Chemistry Faculty of Mathematics and Natural Sciences Universitas Padjadjaran. Jl. Raya Bandung-Sumedang km.21 Jatinangor, Sumedang, West Java, Indonesia 45363Andrographis paniculata or sambiloto is one of the most widely used medicinal herbs in Indonesia. The main bioactive chemical constituent, andrographolide, has been reported to have various pharmacological activities. Besides its function for medical purposes, the sambiloto herbs infusion is frequently taken to maintain health. This study was conducted to determine the bioavailability of sambiloto herbs infusion in rabbit plasma, stomach, and liver, calculated as total andrographolide. Fourteen male New Zealand white rabbits were used in this study. Sambiloto herbs infusion were administered orally at the dose 7.04mL/kg body weight to each rabbit. Blood samples were taken at intervals 0.0; 0.5; 1.5; 2.0; 3.0; and 5.0h after infusion administration. Sambiloto herbs infusion, which are calculated as andrographolide, levels in plasma, stomach, and liver were analyzed by high performance liquid chromatography using C-18 column as stationary phase and a mixture of methanol-double distilled water (60:40) as mobile phase. Bioavailability parameters obtained were Cmax 0.5549µg/mL (in stomach), 0.2136µg/mL (in plasma), 0.0051µg/mL (in liver); while tmax 1h (in stomach), 1.5h (in plasma), 2h (in liver); and AUC 1.7451µg.h/mL (in stomach), 0.434µg.h/mL (in plasma), 0.0038µg.h/mL (in liver). These data showed that in healthy animals, sambiloto herbs infusion was fastly absorbed from the stomach, distributed in the circulation system, and metabolized in the liver, in subsequent process. Sambiloto herbs infusion showed good bioavailability in rabbit. http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/215andrographolideAndrographis paniculatabioavailabilitysambiloto |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jutti Levita Hetsa Himawati , Rizky Vitriani Lukman Melysa Afdila Holis Abdul Holik Nyi Mekar Saptarini Aliya Nur Hasanah Aliya Nur Hasanah As’ari Nawawi Slamet Ibrahim Abdul Mutalib |
spellingShingle |
Jutti Levita Hetsa Himawati , Rizky Vitriani Lukman Melysa Afdila Holis Abdul Holik Nyi Mekar Saptarini Aliya Nur Hasanah Aliya Nur Hasanah As’ari Nawawi Slamet Ibrahim Abdul Mutalib BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT Indonesian Journal of Pharmacy andrographolide Andrographis paniculata bioavailability sambiloto |
author_facet |
Jutti Levita Hetsa Himawati , Rizky Vitriani Lukman Melysa Afdila Holis Abdul Holik Nyi Mekar Saptarini Aliya Nur Hasanah Aliya Nur Hasanah As’ari Nawawi Slamet Ibrahim Abdul Mutalib |
author_sort |
Jutti Levita |
title |
BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT |
title_short |
BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT |
title_full |
BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT |
title_fullStr |
BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT |
title_full_unstemmed |
BIOAVAILABILITY STUDY OF SAMBILOTO (Andrographis paniculata) HERBS INFUSION IN RABBIT |
title_sort |
bioavailability study of sambiloto (andrographis paniculata) herbs infusion in rabbit |
publisher |
Universitas Gadjah Mada |
series |
Indonesian Journal of Pharmacy |
issn |
2338-9427 2338-9486 |
publishDate |
2014-07-01 |
description |
Andrographis paniculata or sambiloto is one of the most
widely used medicinal herbs in Indonesia. The main bioactive
chemical constituent, andrographolide, has been reported to have
various pharmacological activities. Besides its function for medical
purposes, the sambiloto herbs infusion is frequently taken to
maintain health. This study was conducted to determine the
bioavailability of sambiloto herbs infusion in rabbit plasma,
stomach, and liver, calculated as total andrographolide. Fourteen
male New Zealand white rabbits were used in this study. Sambiloto
herbs infusion were administered orally at the dose 7.04mL/kg
body weight to each rabbit. Blood samples were taken at intervals
0.0; 0.5; 1.5; 2.0; 3.0; and 5.0h after infusion administration.
Sambiloto herbs infusion, which are calculated as andrographolide,
levels in plasma, stomach, and liver were analyzed by high
performance liquid chromatography using C-18 column as stationary
phase and a mixture of methanol-double distilled water (60:40) as
mobile phase. Bioavailability parameters obtained were Cmax
0.5549µg/mL (in stomach), 0.2136µg/mL (in plasma), 0.0051µg/mL
(in liver); while tmax 1h (in stomach), 1.5h (in plasma), 2h (in
liver); and AUC 1.7451µg.h/mL (in stomach), 0.434µg.h/mL (in
plasma), 0.0038µg.h/mL (in liver). These data showed that in
healthy animals, sambiloto herbs infusion was fastly absorbed
from the stomach, distributed in the circulation system, and
metabolized in the liver, in subsequent process. Sambiloto herbs
infusion showed good bioavailability in rabbit. |
topic |
andrographolide Andrographis paniculata bioavailability sambiloto |
url |
http://indonesianjpharm.farmasi.ugm.ac.id/index.php/3/article/view/215 |
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