DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period
This study was designed to investigate the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in dairy cattle during the transition period. Blood samples were collected from 4 dairy cattle at 3 weeks before the expected parturition (wk -3...
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Tripoli University
2012-06-01
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doaj-d96631bef901421399b8c08279c124ac2021-10-02T08:37:23ZengTripoli UniversityOpen Veterinary Journal2218-60502012-06-0126568DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition periodS. OikawaD. EndohM. TharwatThis study was designed to investigate the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in dairy cattle during the transition period. Blood samples were collected from 4 dairy cattle at 3 weeks before the expected parturition (wk -3), parturition (wk 0) and 3 weeks after parturition (wk +3). The DNA damage of PBMC and PMN was evaluated based on the comet assay using visual scoring (arbitrary units). Undamaged DNA remained within the core (score 0) and the broken DNA migrated from the core towards the anode forming the tail of a comet (scores 1-4). Significantly higher scores in PBMC at wk 0 and wk +3 were observed compared with those in PMN although there were no significant changes of scores in either cell type during the experimental period. It is suggested that the apoptotic rate of PBMC is accelerated compared with that of PMC during the transition period.http://www.openveterinaryjournal.com/2012/OVJ-041-04-12%20M.%20Tharwat%20et%20al.pdfApoptosisComet assayCowLeukocytesTransition period. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
S. Oikawa D. Endoh M. Tharwat |
spellingShingle |
S. Oikawa D. Endoh M. Tharwat DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period Open Veterinary Journal Apoptosis Comet assay Cow Leukocytes Transition period. |
author_facet |
S. Oikawa D. Endoh M. Tharwat |
author_sort |
S. Oikawa |
title |
DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period |
title_short |
DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period |
title_full |
DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period |
title_fullStr |
DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period |
title_full_unstemmed |
DNA damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period |
title_sort |
dna damage in peripheral blood mononuclear cells and neutrophils of dairy cows during the transition period |
publisher |
Tripoli University |
series |
Open Veterinary Journal |
issn |
2218-6050 |
publishDate |
2012-06-01 |
description |
This study was designed to investigate the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in dairy cattle during the transition period. Blood samples were collected from 4 dairy cattle at 3 weeks before the expected parturition (wk -3), parturition (wk 0) and 3 weeks after parturition (wk +3). The DNA damage of PBMC and PMN was evaluated based on the comet assay using visual scoring (arbitrary units). Undamaged DNA remained within the core (score 0) and the broken DNA migrated from the core towards the anode forming the tail of a comet (scores 1-4). Significantly higher scores in PBMC at wk 0 and wk +3 were observed compared with those in PMN although there were no significant changes of scores in either cell type during the experimental period. It is suggested that the apoptotic rate of PBMC is accelerated compared with that of PMC during the transition period. |
topic |
Apoptosis Comet assay Cow Leukocytes Transition period. |
url |
http://www.openveterinaryjournal.com/2012/OVJ-041-04-12%20M.%20Tharwat%20et%20al.pdf |
work_keys_str_mv |
AT soikawa dnadamageinperipheralbloodmononuclearcellsandneutrophilsofdairycowsduringthetransitionperiod AT dendoh dnadamageinperipheralbloodmononuclearcellsandneutrophilsofdairycowsduringthetransitionperiod AT mtharwat dnadamageinperipheralbloodmononuclearcellsandneutrophilsofdairycowsduringthetransitionperiod |
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1716856875610275840 |