Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite

The objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydrox...

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Main Authors: Paulina-Guadalupe Miranda-Meléndez, Gabriel-Alejandro Martínez-Castañón, Nereyda Niño-Martínez, Nuria Patiño-Marín, Miguel-Ángel Casillas-Santana, Brenda-Erendida Castillo-Silva, Facundo Ruiz
Format: Article
Language:English
Published: Hindawi Limited 2016-01-01
Series:Bioinorganic Chemistry and Applications
Online Access:http://dx.doi.org/10.1155/2016/1057260
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spelling doaj-d9828dfd78e948f787262b46c0d985792020-11-25T02:28:06ZengHindawi LimitedBioinorganic Chemistry and Applications1565-36331687-479X2016-01-01201610.1155/2016/10572601057260Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped NanohydroxyapatitePaulina-Guadalupe Miranda-Meléndez0Gabriel-Alejandro Martínez-Castañón1Nereyda Niño-Martínez2Nuria Patiño-Marín3Miguel-Ángel Casillas-Santana4Brenda-Erendida Castillo-Silva5Facundo Ruiz6Doctorado Institucional en Ingeniería y Ciencia de los Materiales, Universidad Autónoma de San Luis Potosí, Av. Salvador Nava s/n, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado Institucional en Ingeniería y Ciencia de los Materiales, Universidad Autónoma de San Luis Potosí, Av. Salvador Nava s/n, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoDoctorado en Ciencias Odontológicas, Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Av. Dr. Manuel Nava 2, Zona Universitaria, 78290 San Luis Potosí, SLP, MexicoThe objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydroxyapatite was doped with 3, 5, 10, and 20 wt% of europium. The obtained samples were characterized after they were dried at 80°C and hydrothermal treated at 120°C by 2 hours. The samples were analyzed by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and photoluminescence. Also, impregnation and release of 5-fluorouracil were assessed in PBS. The toxicity effects of all samples were studied using viability assays on human fibroblasts cells (HGF-1) in vitro. The sizes of the crystallites were about 10–70 nm with irregular morphology and present the phase corresponding to the JCPDS card 9–0432 for hydroxyapatite. The results of the toxicity experiments indicated that doped and undoped powders are biocompatible with fibroblasts cells. Hydroxyapatite samples doped with 5% of europium and loaded with 5-fluorouracil release almost 7 mg/L of the drug after 60 minutes in PBS and decrease the viability of HeLa cells after 24 hours.http://dx.doi.org/10.1155/2016/1057260
collection DOAJ
language English
format Article
sources DOAJ
author Paulina-Guadalupe Miranda-Meléndez
Gabriel-Alejandro Martínez-Castañón
Nereyda Niño-Martínez
Nuria Patiño-Marín
Miguel-Ángel Casillas-Santana
Brenda-Erendida Castillo-Silva
Facundo Ruiz
spellingShingle Paulina-Guadalupe Miranda-Meléndez
Gabriel-Alejandro Martínez-Castañón
Nereyda Niño-Martínez
Nuria Patiño-Marín
Miguel-Ángel Casillas-Santana
Brenda-Erendida Castillo-Silva
Facundo Ruiz
Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
Bioinorganic Chemistry and Applications
author_facet Paulina-Guadalupe Miranda-Meléndez
Gabriel-Alejandro Martínez-Castañón
Nereyda Niño-Martínez
Nuria Patiño-Marín
Miguel-Ángel Casillas-Santana
Brenda-Erendida Castillo-Silva
Facundo Ruiz
author_sort Paulina-Guadalupe Miranda-Meléndez
title Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
title_short Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
title_full Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
title_fullStr Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
title_full_unstemmed Facile Synthesis, Characterization, and Cytotoxic Activity of Europium-Doped Nanohydroxyapatite
title_sort facile synthesis, characterization, and cytotoxic activity of europium-doped nanohydroxyapatite
publisher Hindawi Limited
series Bioinorganic Chemistry and Applications
issn 1565-3633
1687-479X
publishDate 2016-01-01
description The objective of this study was to synthetize europium-doped nanohydroxyapatite using a simple aqueous precipitation method and, thereafter, characterize and impregnate selected samples with 5-fluorouracil in order to explore the properties and the releasing capacity of this material. The nanohydroxyapatite was doped with 3, 5, 10, and 20 wt% of europium. The obtained samples were characterized after they were dried at 80°C and hydrothermal treated at 120°C by 2 hours. The samples were analyzed by transmission electron microscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and photoluminescence. Also, impregnation and release of 5-fluorouracil were assessed in PBS. The toxicity effects of all samples were studied using viability assays on human fibroblasts cells (HGF-1) in vitro. The sizes of the crystallites were about 10–70 nm with irregular morphology and present the phase corresponding to the JCPDS card 9–0432 for hydroxyapatite. The results of the toxicity experiments indicated that doped and undoped powders are biocompatible with fibroblasts cells. Hydroxyapatite samples doped with 5% of europium and loaded with 5-fluorouracil release almost 7 mg/L of the drug after 60 minutes in PBS and decrease the viability of HeLa cells after 24 hours.
url http://dx.doi.org/10.1155/2016/1057260
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