Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade
Shading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus (SMV) is a biotic factor that limits the yield and quality of soybean. However, little is known about the defense response of soybean to SMV in the shade. Thus, i...
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doaj-d98daf9cdc484b1986f88f6d03fa8b282020-11-24T20:42:54ZengMDPI AGViruses1999-49152019-08-0111979310.3390/v11090793v11090793Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the ShadeLei Zhang0Jing Shang1Wenming Wang2Junbo Du3Kai Li4Xiaoling Wu5Liang Yu6Chunyan Liu7Muhammad Ibrahim Khaskheli8Wenyu Yang9Sichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaNational Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing 210095, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaSichuan Engineering Research Center for Crop Strip Intercropping System, College of Agronomy and Key Laboratory for Major Crop Diseases, Sichuan Agricultural University, Chengdu 611130, ChinaShading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus (SMV) is a biotic factor that limits the yield and quality of soybean. However, little is known about the defense response of soybean to SMV in the shade. Thus, in the current study, both intensity and quality (red:far-red, R:FR) of the light were changed to simulate the shaded environment and comparative transcriptome analysis was performed. Morphologically, plant growth was inhibited by SMV, which decreased 35.93% of plant height and 8.97% of stem diameter in the shade. A total of 3548 and 4319 differentially expressed genes (DEGs) were identified in soybean plants infected with SMV under normal light and in the shade. Enrichment analysis showed that the plant defense-related genes were upregulated under normal light but downregulated in the shade. Pathways that were repressed include plant-pathogen interaction, secondary metabolism, sugar metabolism, and vitamin metabolism. In addition, genes associated with signaling pathways such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH) were also downregulated in the shade. A qRT-PCR assay of 15 DEGs was performed to confirm transcriptome results. According to our knowledge, this is the first report on soybean response to dual stress factors. These results provide insights into the molecular mechanisms in which soybean plants were infected with SMV in the shade.https://www.mdpi.com/1999-4915/11/9/793soybeansoybean mosaic virusshadingRNA-Seqplant-pathogen interaction |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Lei Zhang Jing Shang Wenming Wang Junbo Du Kai Li Xiaoling Wu Liang Yu Chunyan Liu Muhammad Ibrahim Khaskheli Wenyu Yang |
spellingShingle |
Lei Zhang Jing Shang Wenming Wang Junbo Du Kai Li Xiaoling Wu Liang Yu Chunyan Liu Muhammad Ibrahim Khaskheli Wenyu Yang Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade Viruses soybean soybean mosaic virus shading RNA-Seq plant-pathogen interaction |
author_facet |
Lei Zhang Jing Shang Wenming Wang Junbo Du Kai Li Xiaoling Wu Liang Yu Chunyan Liu Muhammad Ibrahim Khaskheli Wenyu Yang |
author_sort |
Lei Zhang |
title |
Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade |
title_short |
Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade |
title_full |
Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade |
title_fullStr |
Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade |
title_full_unstemmed |
Comparison of Transcriptome Differences in Soybean Response to Soybean Mosaic Virus under Normal Light and in the Shade |
title_sort |
comparison of transcriptome differences in soybean response to soybean mosaic virus under normal light and in the shade |
publisher |
MDPI AG |
series |
Viruses |
issn |
1999-4915 |
publishDate |
2019-08-01 |
description |
Shading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus (SMV) is a biotic factor that limits the yield and quality of soybean. However, little is known about the defense response of soybean to SMV in the shade. Thus, in the current study, both intensity and quality (red:far-red, R:FR) of the light were changed to simulate the shaded environment and comparative transcriptome analysis was performed. Morphologically, plant growth was inhibited by SMV, which decreased 35.93% of plant height and 8.97% of stem diameter in the shade. A total of 3548 and 4319 differentially expressed genes (DEGs) were identified in soybean plants infected with SMV under normal light and in the shade. Enrichment analysis showed that the plant defense-related genes were upregulated under normal light but downregulated in the shade. Pathways that were repressed include plant-pathogen interaction, secondary metabolism, sugar metabolism, and vitamin metabolism. In addition, genes associated with signaling pathways such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH) were also downregulated in the shade. A qRT-PCR assay of 15 DEGs was performed to confirm transcriptome results. According to our knowledge, this is the first report on soybean response to dual stress factors. These results provide insights into the molecular mechanisms in which soybean plants were infected with SMV in the shade. |
topic |
soybean soybean mosaic virus shading RNA-Seq plant-pathogen interaction |
url |
https://www.mdpi.com/1999-4915/11/9/793 |
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