The effect of antigen retrieval on cells fixed in 10% neutral buffered formalin followed by transfer to 70% ethanol.
Fixation in 10% neutral buffered formalin prior to transfer to 70% ethanol for one week has been shown to adequately preserve immunorecognition of PCNA, cytokeratins AE1/AE3 and EGFr. This study investigated whether 12 hrs fixation in 10% NBF plus transfer to 70% ethanol for 4 weeks would similarly...
Main Authors: | Dennis Otali, Qinghua He, William E Grizzle |
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Format: | Article |
Language: | English |
Published: |
Public Library of Science (PLoS)
2013-01-01
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Series: | PLoS ONE |
Online Access: | http://europepmc.org/articles/PMC3866107?pdf=render |
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