Characterization of the CD14++CD16+ monocyte population in human bone marrow.
Numerous studies have divided blood monocytes according to their expression of the surface markers CD14 and CD16 into following subsets: classical CD14(++)CD16(-), intermediate CD14(++)CD16(+) and nonclassical CD14(+)CD16(++) monocytes. These subsets differ in phenotype and function and are further...
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doaj-d9b76f352a3d4ffb803da19c211337df2020-11-25T01:48:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01911e11214010.1371/journal.pone.0112140Characterization of the CD14++CD16+ monocyte population in human bone marrow.Manuela MandlSusanne SchmitzChristian WeberMichael HristovNumerous studies have divided blood monocytes according to their expression of the surface markers CD14 and CD16 into following subsets: classical CD14(++)CD16(-), intermediate CD14(++)CD16(+) and nonclassical CD14(+)CD16(++) monocytes. These subsets differ in phenotype and function and are further correlated to cardiovascular disease, inflammation and cancer. However, the CD14/CD16 nature of resident monocytes in human bone marrow remains largely unknown. In the present study, we identified a major population of CD14(++)CD16(+) monocytes by using cryopreserved bone marrow mononuclear cells from healthy donors. These cells express essential monocyte-related antigens and chemokine receptors such as CD11a, CD18, CD44, HLA-DR, Ccr2, Ccr5, Cx3cr1, Cxcr2 and Cxcr4. Notably, the expression of Ccr2 was inducible during culture. Furthermore, sorted CD14(++)CD16(+) bone marrow cells show typical macrophage morphology, phagocytic activity, angiogenic features and generation of intracellular oxygen species. Side-by-side comparison of the chemokine receptor profile with unpaired blood samples also demonstrated that these rather premature medullar monocytes mainly match the phenotype of intermediate and partially of (non)classical monocytes. Together, human monocytes obviously acquire their definitive CD14/CD16 signature in the bloodstream and the medullar monocytes probably transform into CD14(++)CD16- and CD14(+)CD16(++) subsets which appear enriched in the periphery.http://europepmc.org/articles/PMC4219836?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Manuela Mandl Susanne Schmitz Christian Weber Michael Hristov |
spellingShingle |
Manuela Mandl Susanne Schmitz Christian Weber Michael Hristov Characterization of the CD14++CD16+ monocyte population in human bone marrow. PLoS ONE |
author_facet |
Manuela Mandl Susanne Schmitz Christian Weber Michael Hristov |
author_sort |
Manuela Mandl |
title |
Characterization of the CD14++CD16+ monocyte population in human bone marrow. |
title_short |
Characterization of the CD14++CD16+ monocyte population in human bone marrow. |
title_full |
Characterization of the CD14++CD16+ monocyte population in human bone marrow. |
title_fullStr |
Characterization of the CD14++CD16+ monocyte population in human bone marrow. |
title_full_unstemmed |
Characterization of the CD14++CD16+ monocyte population in human bone marrow. |
title_sort |
characterization of the cd14++cd16+ monocyte population in human bone marrow. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Numerous studies have divided blood monocytes according to their expression of the surface markers CD14 and CD16 into following subsets: classical CD14(++)CD16(-), intermediate CD14(++)CD16(+) and nonclassical CD14(+)CD16(++) monocytes. These subsets differ in phenotype and function and are further correlated to cardiovascular disease, inflammation and cancer. However, the CD14/CD16 nature of resident monocytes in human bone marrow remains largely unknown. In the present study, we identified a major population of CD14(++)CD16(+) monocytes by using cryopreserved bone marrow mononuclear cells from healthy donors. These cells express essential monocyte-related antigens and chemokine receptors such as CD11a, CD18, CD44, HLA-DR, Ccr2, Ccr5, Cx3cr1, Cxcr2 and Cxcr4. Notably, the expression of Ccr2 was inducible during culture. Furthermore, sorted CD14(++)CD16(+) bone marrow cells show typical macrophage morphology, phagocytic activity, angiogenic features and generation of intracellular oxygen species. Side-by-side comparison of the chemokine receptor profile with unpaired blood samples also demonstrated that these rather premature medullar monocytes mainly match the phenotype of intermediate and partially of (non)classical monocytes. Together, human monocytes obviously acquire their definitive CD14/CD16 signature in the bloodstream and the medullar monocytes probably transform into CD14(++)CD16- and CD14(+)CD16(++) subsets which appear enriched in the periphery. |
url |
http://europepmc.org/articles/PMC4219836?pdf=render |
work_keys_str_mv |
AT manuelamandl characterizationofthecd14cd16monocytepopulationinhumanbonemarrow AT susanneschmitz characterizationofthecd14cd16monocytepopulationinhumanbonemarrow AT christianweber characterizationofthecd14cd16monocytepopulationinhumanbonemarrow AT michaelhristov characterizationofthecd14cd16monocytepopulationinhumanbonemarrow |
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