Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity
Bacterial biofilms adhere to all oral surfaces and may alter or degrade them. For investigations of the oral biofilm, growing on new restorative dental biomaterials, sterilized dental enamel surfaces as natural, control, and reference materials are used. A novel method for disinfection and steriliza...
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2019-02-01
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doaj-d9c0731248f04eb4b87ff5000d5d49c42020-11-25T00:27:20ZengMDPI AGCoatings2079-64122019-02-01929910.3390/coatings9020099coatings9020099Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral CavityElla A. Naumova0Alexander-Simon Engel1Hagen Tizian Kranz2Marvin Schneider3Jan Tietze4Thomas Dittmar5Marcel Fiebrandt6Katharina Stapelmann7Andree Piwowarczyk8Thorsten Kuczius9Wolfgang H. Arnold10Department of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyDepartment of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyDepartment of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyDepartment of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyDepartment of Biological and Material Sciences in Dentistry, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyDepartment of Immunology, Center for Biomedical Education and Research, Faculty of Health, Witten/Herdecke University, 58453 Witten, GermanyFaculty of Electrical Engineering and Information Technology, Institute for Electrical Engineering and Plasma Technology, Ruhr University Bochum, 44801 Bochum, GermanyFaculty of Electrical Engineering and Information Technology, Institute for Electrical Engineering and Plasma Technology, Ruhr University Bochum, 44801 Bochum, GermanyDepartment of Prosthodontics and Dental Technology, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyInstitute for Hygiene, Faculty of Medicine, Westfälische Wilhelms-University Muenster, Robert Koch Straße 41, 48149 Muenster, GermanySchool of Dentistry, Faculty of Health, Witten/Herdecke University, Alfred Herrhausenstrasse 44, 58455 Witten, GermanyBacterial biofilms adhere to all oral surfaces and may alter or degrade them. For investigations of the oral biofilm, growing on new restorative dental biomaterials, sterilized dental enamel surfaces as natural, control, and reference materials are used. A novel method for disinfection and sterilization of surfaces is low-pressure plasma (LPP) sterilization, which is a nondestructive and nontoxic technology. The roughness of the dental enamel surface was determined before and after LPP sterilization. Enamel discs were placed in dental splints and worn for five days in vivo. Oral biofilm was fixed for scanning electron microscopy (SEM). Biofilms growing in vitro were characterized microbiologically before and after sterilization and examined by confocal laser scanning microscopy (CLSM). Microbiology demonstrated that various bacterial strains were present in the biofilms. SEM showed multiple layers of densely packed bacteria, and CLSM demonstrated that the biofilm contained live and dead bacteria. After LPP sterilization, no biofilm could be detected, and the enamel surface remained unaltered. It may be concluded that LPP sterilization is an effective, nondestructive method for disinfection of enamel before application in the oral cavity. LPP sterilization may be suitable for sterilization of dental materials without altering their surfaces.https://www.mdpi.com/2079-6412/9/2/99plasmasterilizationbiofilmoral biofilm |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ella A. Naumova Alexander-Simon Engel Hagen Tizian Kranz Marvin Schneider Jan Tietze Thomas Dittmar Marcel Fiebrandt Katharina Stapelmann Andree Piwowarczyk Thorsten Kuczius Wolfgang H. Arnold |
spellingShingle |
Ella A. Naumova Alexander-Simon Engel Hagen Tizian Kranz Marvin Schneider Jan Tietze Thomas Dittmar Marcel Fiebrandt Katharina Stapelmann Andree Piwowarczyk Thorsten Kuczius Wolfgang H. Arnold Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity Coatings plasma sterilization biofilm oral biofilm |
author_facet |
Ella A. Naumova Alexander-Simon Engel Hagen Tizian Kranz Marvin Schneider Jan Tietze Thomas Dittmar Marcel Fiebrandt Katharina Stapelmann Andree Piwowarczyk Thorsten Kuczius Wolfgang H. Arnold |
author_sort |
Ella A. Naumova |
title |
Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity |
title_short |
Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity |
title_full |
Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity |
title_fullStr |
Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity |
title_full_unstemmed |
Low-Pressure Plasma Sterilization for Test Specimens to be Worn on Splints in the Oral Cavity |
title_sort |
low-pressure plasma sterilization for test specimens to be worn on splints in the oral cavity |
publisher |
MDPI AG |
series |
Coatings |
issn |
2079-6412 |
publishDate |
2019-02-01 |
description |
Bacterial biofilms adhere to all oral surfaces and may alter or degrade them. For investigations of the oral biofilm, growing on new restorative dental biomaterials, sterilized dental enamel surfaces as natural, control, and reference materials are used. A novel method for disinfection and sterilization of surfaces is low-pressure plasma (LPP) sterilization, which is a nondestructive and nontoxic technology. The roughness of the dental enamel surface was determined before and after LPP sterilization. Enamel discs were placed in dental splints and worn for five days in vivo. Oral biofilm was fixed for scanning electron microscopy (SEM). Biofilms growing in vitro were characterized microbiologically before and after sterilization and examined by confocal laser scanning microscopy (CLSM). Microbiology demonstrated that various bacterial strains were present in the biofilms. SEM showed multiple layers of densely packed bacteria, and CLSM demonstrated that the biofilm contained live and dead bacteria. After LPP sterilization, no biofilm could be detected, and the enamel surface remained unaltered. It may be concluded that LPP sterilization is an effective, nondestructive method for disinfection of enamel before application in the oral cavity. LPP sterilization may be suitable for sterilization of dental materials without altering their surfaces. |
topic |
plasma sterilization biofilm oral biofilm |
url |
https://www.mdpi.com/2079-6412/9/2/99 |
work_keys_str_mv |
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