The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition
The maternal-to-zygotic transition (MZT) is a conserved step in animal development, where control is passed from the maternal to the zygotic genome. Although the MZT is typically considered from its impact on the transcriptome, we previously found that three maternally deposited Drosophila RNA-bindi...
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doaj-d9c1b57f26f64ef38cdd74811f0c81522021-05-05T21:14:13ZengeLife Sciences Publications LtdeLife2050-084X2020-06-01910.7554/eLife.53889The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transitionMichael Zavortink0https://orcid.org/0000-0002-7967-1506Lauren N Rutt1https://orcid.org/0000-0001-9861-207XSvetlana Dzitoyeva2https://orcid.org/0000-0002-5471-600XJesslyn C Henriksen3https://orcid.org/0000-0002-7371-2346Chloe Barrington4https://orcid.org/0000-0002-3884-102XDanielle Y Bilodeau5https://orcid.org/0000-0002-8086-4233Miranda Wang6https://orcid.org/0000-0002-8163-4341Xiao Xiao Lily Chen7https://orcid.org/0000-0003-1529-0568Olivia S Rissland8https://orcid.org/0000-0002-2619-6019University of Colorado School of Medicine, Aurora, United StatesUniversity of Colorado School of Medicine, Aurora, United StatesUniversity of Colorado School of Medicine, Aurora, United StatesUniversity of Colorado School of Medicine, Aurora, United StatesUniversity of Colorado School of Medicine, Aurora, United StatesUniversity of Colorado School of Medicine, Aurora, United StatesUniversity of Toronto, Toronto, CanadaUniversity of Toronto, Toronto, CanadaUniversity of Colorado School of Medicine, Aurora, United StatesThe maternal-to-zygotic transition (MZT) is a conserved step in animal development, where control is passed from the maternal to the zygotic genome. Although the MZT is typically considered from its impact on the transcriptome, we previously found that three maternally deposited Drosophila RNA-binding proteins (ME31B, Trailer Hitch [TRAL], and Cup) are also cleared during the MZT by unknown mechanisms. Here, we show that these proteins are degraded by the ubiquitin-proteasome system. Marie Kondo, an E2 conjugating enzyme, and the E3 CTLH ligase are required for the destruction of ME31B, TRAL, and Cup. Structure modeling of the Drosophila CTLH complex suggests that substrate recognition is different than orthologous complexes. Despite occurring hours earlier, egg activation mediates clearance of these proteins through the Pan Gu kinase, which stimulates translation of Kdo mRNA. Clearance of the maternal protein dowry thus appears to be a coordinated, but as-yet underappreciated, aspect of the MZT.https://elifesciences.org/articles/53889maternal to zygotic transitionRNA binding proteinsubiquitin-proteasome systempost-transcriptional regulation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Michael Zavortink Lauren N Rutt Svetlana Dzitoyeva Jesslyn C Henriksen Chloe Barrington Danielle Y Bilodeau Miranda Wang Xiao Xiao Lily Chen Olivia S Rissland |
spellingShingle |
Michael Zavortink Lauren N Rutt Svetlana Dzitoyeva Jesslyn C Henriksen Chloe Barrington Danielle Y Bilodeau Miranda Wang Xiao Xiao Lily Chen Olivia S Rissland The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition eLife maternal to zygotic transition RNA binding proteins ubiquitin-proteasome system post-transcriptional regulation |
author_facet |
Michael Zavortink Lauren N Rutt Svetlana Dzitoyeva Jesslyn C Henriksen Chloe Barrington Danielle Y Bilodeau Miranda Wang Xiao Xiao Lily Chen Olivia S Rissland |
author_sort |
Michael Zavortink |
title |
The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition |
title_short |
The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition |
title_full |
The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition |
title_fullStr |
The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition |
title_full_unstemmed |
The E2 Marie Kondo and the CTLH E3 ligase clear deposited RNA binding proteins during the maternal-to-zygotic transition |
title_sort |
e2 marie kondo and the ctlh e3 ligase clear deposited rna binding proteins during the maternal-to-zygotic transition |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-06-01 |
description |
The maternal-to-zygotic transition (MZT) is a conserved step in animal development, where control is passed from the maternal to the zygotic genome. Although the MZT is typically considered from its impact on the transcriptome, we previously found that three maternally deposited Drosophila RNA-binding proteins (ME31B, Trailer Hitch [TRAL], and Cup) are also cleared during the MZT by unknown mechanisms. Here, we show that these proteins are degraded by the ubiquitin-proteasome system. Marie Kondo, an E2 conjugating enzyme, and the E3 CTLH ligase are required for the destruction of ME31B, TRAL, and Cup. Structure modeling of the Drosophila CTLH complex suggests that substrate recognition is different than orthologous complexes. Despite occurring hours earlier, egg activation mediates clearance of these proteins through the Pan Gu kinase, which stimulates translation of Kdo mRNA. Clearance of the maternal protein dowry thus appears to be a coordinated, but as-yet underappreciated, aspect of the MZT. |
topic |
maternal to zygotic transition RNA binding proteins ubiquitin-proteasome system post-transcriptional regulation |
url |
https://elifesciences.org/articles/53889 |
work_keys_str_mv |
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