Cryopreservation of semen of Thailand tilapia (Oreochromis spp) fed diet with different oil soucer

• Main Authors: Rodrigo Diana Navarro, Fernanda Keley Silva Pereira Navarro, Viviane Oliveira Felizardo, Luis David Solis Murgas, Marcelo Maia Pereira
• Format: Article
• Language: English
• Published: Universidade Estadual de Maringá 2014-02-01
• Series: Acta Scientiarum: Technology
• Subjects: freeze, cryoprotectant, DMSO, Methanol
• Online Access: http://186.233.154.254/ojs/index.php/ActaSciTechnol/article/view/20076
• ISSN: 1806-2563; 1807-8664

This study evaluated the quality of sperm cryopreserved with dimethylsulfoxide (DMSO) or methanol, of Thailand tilapia (Oreochromis spp) fed diet with different oilsources. Methods: For this, 80 Thailand breeding tilapia fed diets with four sources of oil (T1: linseed oil, T2: soybean oil, T3: fish oil, T4: corn oil). No significant difference was found for the final weight, total length, rate and duration of motility of fresh sperm in the different treatments. Significant differences were observed in fish, corn and linseed oil treatment on the rate of motility relative to other different oil sources with the cryoprotectant DMSO prior to freezing.  After thawing, fish subjected to a diet supplemented with fish oil presented a significantly higher rate and duration of sperm motility. Nevertheless, for better reproductive parameters after freezing, it is recommended the use of fish oil associated with cryoprotectant methanol.