Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis
Purpose. Bisphenol A (BPA) is found in many plastic products and is thus a common environmental endocrine disruptor. Plastic-related health problems, including allergic diseases, are attracting increasing attention. However, few experimental studies have explored the effect of BPA on allergic rhinit...
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doaj-d9faddd751c54146ada7734b6a58cac82020-11-25T02:35:49ZengHindawi LimitedJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/75731037573103Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic RhinitisYunxiu Wang0Zhiwei Cao1He Zhao2Yaoyao Ren3Liying Hao4Zhaowei Gu5Department of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City, 110004 Liaoning Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City, 110004 Liaoning Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City, 110004 Liaoning Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City, 110004 Liaoning Province, ChinaDepartment of Pharmaceutical Toxicology, School of Pharmacy, China Medical University, Shenyang City, 110122 Liaoning Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, Shengjing Hospital of China Medical University, Shenyang City, 110004 Liaoning Province, ChinaPurpose. Bisphenol A (BPA) is found in many plastic products and is thus a common environmental endocrine disruptor. Plastic-related health problems, including allergic diseases, are attracting increasing attention. However, few experimental studies have explored the effect of BPA on allergic rhinitis (AR). We explore whether BPA was directly related to the allergic inflammation induced by ovalbumin (OVA) in AR mice. Methods. We first constructed OVA-induced mouse model, and after BPA administration, we evaluated nasal symptoms and measured the serum OVA-specific IgE levels by ELISA. Th2 and Treg-related cytokines of nasal mucosa were measured by cytometric bead array. Th2 and Treg-specific transcription factor levels were assayed by PCR. The proportions of CD3+CD4+IL-4+Th2 and CD4+Helios+Foxp3+ T cells (Tregs) in spleen tissue were determined by flow cytometry. Results. Compared to OVA-only-induced mice, BPA addition increased nasal symptoms and serum OVA-specific IgE levels. OVA and BPA coexposure significantly increased IL-4 and IL-13 protein levels compared to those after OVA exposure alone. BPA plus OVA tended to decrease the IL-10 protein levels compared to those after OVA alone. Coexposure to OVA and BPA significantly increased the GATA-3-encoding mRNA level, and decreased the levels of mRNAs encoding Foxp3 and Helios, compared to those after OVA exposure alone. BPA increased the Th2 cell proportion, and decreased that of Tregs, compared to the levels with OVA alone. Conclusion. BPA exerted negative effects by exacerbating AR allergic symptoms, increasing serum OVA-specific IgE levels, and compromising Th2 and Treg responses.http://dx.doi.org/10.1155/2020/7573103 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yunxiu Wang Zhiwei Cao He Zhao Yaoyao Ren Liying Hao Zhaowei Gu |
spellingShingle |
Yunxiu Wang Zhiwei Cao He Zhao Yaoyao Ren Liying Hao Zhaowei Gu Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis Journal of Immunology Research |
author_facet |
Yunxiu Wang Zhiwei Cao He Zhao Yaoyao Ren Liying Hao Zhaowei Gu |
author_sort |
Yunxiu Wang |
title |
Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis |
title_short |
Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis |
title_full |
Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis |
title_fullStr |
Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis |
title_full_unstemmed |
Bisphenol A Exacerbates Allergic Inflammation in an Ovalbumin-Induced Mouse Model of Allergic Rhinitis |
title_sort |
bisphenol a exacerbates allergic inflammation in an ovalbumin-induced mouse model of allergic rhinitis |
publisher |
Hindawi Limited |
series |
Journal of Immunology Research |
issn |
2314-8861 2314-7156 |
publishDate |
2020-01-01 |
description |
Purpose. Bisphenol A (BPA) is found in many plastic products and is thus a common environmental endocrine disruptor. Plastic-related health problems, including allergic diseases, are attracting increasing attention. However, few experimental studies have explored the effect of BPA on allergic rhinitis (AR). We explore whether BPA was directly related to the allergic inflammation induced by ovalbumin (OVA) in AR mice. Methods. We first constructed OVA-induced mouse model, and after BPA administration, we evaluated nasal symptoms and measured the serum OVA-specific IgE levels by ELISA. Th2 and Treg-related cytokines of nasal mucosa were measured by cytometric bead array. Th2 and Treg-specific transcription factor levels were assayed by PCR. The proportions of CD3+CD4+IL-4+Th2 and CD4+Helios+Foxp3+ T cells (Tregs) in spleen tissue were determined by flow cytometry. Results. Compared to OVA-only-induced mice, BPA addition increased nasal symptoms and serum OVA-specific IgE levels. OVA and BPA coexposure significantly increased IL-4 and IL-13 protein levels compared to those after OVA exposure alone. BPA plus OVA tended to decrease the IL-10 protein levels compared to those after OVA alone. Coexposure to OVA and BPA significantly increased the GATA-3-encoding mRNA level, and decreased the levels of mRNAs encoding Foxp3 and Helios, compared to those after OVA exposure alone. BPA increased the Th2 cell proportion, and decreased that of Tregs, compared to the levels with OVA alone. Conclusion. BPA exerted negative effects by exacerbating AR allergic symptoms, increasing serum OVA-specific IgE levels, and compromising Th2 and Treg responses. |
url |
http://dx.doi.org/10.1155/2020/7573103 |
work_keys_str_mv |
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