In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection

• Main Authors: Asri Insiana Putri, Mohammad Na'iem, Sapto Indrioko, Sri Rahayu, Ari Indrianto
• Format: Article
• Language: English
• Published: Universitas Gadjah Mada, Yogyakarta 2014-12-01
• Series: Indonesian Journal of Biotechnology
• Subjects: F. moluccana; U. tepperianum; gall rust; in vitro selection
• Online Access: https://jurnal.ugm.ac.id/ijbiotech/article/view/9301
• ISSN: 0853-8654; 2089-2241

In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds from two different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material. Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum (Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets in vitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The mean of fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of fresh weight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins and quantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attacked and un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. It is notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminants of pathogenecity. While the highest total phenolic compound were contained in gall rust extract (2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44 %). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U. tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use as media selection in vitro.