In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)
The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiolo...
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Sociedade Brasileira de Fruticultura
2013-12-01
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doaj-da0d933759b144e096ab935141cf9fb32020-11-24T23:52:17ZengSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura1806-99672013-12-013541116112610.1590/S0100-29452013000400023S0100-29452013000400023In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.)Taliane Leila Soares0Onildo Nunes de Jesus1Janay Almeida dos Santos-Serejo2Eder Jorge de Oliveira3EmbrapaEmbrapaEmbrapaEmbrapaThe use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000400023&lng=en&tlng=enMaracujazeirogerminação de pólenanálise histoquímicaespécies silvestres |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Taliane Leila Soares Onildo Nunes de Jesus Janay Almeida dos Santos-Serejo Eder Jorge de Oliveira |
spellingShingle |
Taliane Leila Soares Onildo Nunes de Jesus Janay Almeida dos Santos-Serejo Eder Jorge de Oliveira In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.) Revista Brasileira de Fruticultura Maracujazeiro germinação de pólen análise histoquímica espécies silvestres |
author_facet |
Taliane Leila Soares Onildo Nunes de Jesus Janay Almeida dos Santos-Serejo Eder Jorge de Oliveira |
author_sort |
Taliane Leila Soares |
title |
In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.) |
title_short |
In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.) |
title_full |
In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.) |
title_fullStr |
In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.) |
title_full_unstemmed |
In vitro pollen germination and pollen viability in passion fruit (Passiflora spp.) |
title_sort |
in vitro pollen germination and pollen viability in passion fruit (passiflora spp.) |
publisher |
Sociedade Brasileira de Fruticultura |
series |
Revista Brasileira de Fruticultura |
issn |
1806-9967 |
publishDate |
2013-12-01 |
description |
The use of Passiflora species for ornamental purposes has been recently developed, but little is known about pollen viability and the potential for crossing different species. The objective of this study was to evaluate the pollen viability of six Passiflora species collected from different physiological stages of development through in vitro germination and histochemical analysis using dyes. The pollen was collected in three stages (pre-anthesis, anthesis and post-anthesis). Three compositions of culture medium were used to evaluate the in vitro germination, and two dyes (2,3,5-triphenyltetrazolium chloride, or TTC, and Lugol's solution) were used for the histochemical analysis. The culture medium containing 0.03% Ca(NO3) 4H2O, 0.02% of Mg(SO4 ).7H2O, 0.01% of KNO3, 0,01% of H3BO3, 15% sucrose, and 0.8% agar, pH 7.0, showed a higher percentage of pollen grains germinated. Anthesis is the best time to collect pollen because it promotes high viability and germination. The Lugol's solution and TTC dye overestimated the viability of pollen, as all accessions showed high viability indices when compared with the results obtained in vitro. |
topic |
Maracujazeiro germinação de pólen análise histoquímica espécies silvestres |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452013000400023&lng=en&tlng=en |
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