First report of <i>Enterocytozoon</i> bieneusi and <i>Encephalitozoon intestinalis</i> infection of wild mice in Slovakia

• Main Authors: Oľga Danišová, Alexandra Valenčáková, Michal Stanko, Lenka Luptáková, Antónia Hasajová
• Format: Article
• Language: English
• Published: Institute of Rural Health 2015-05-01
• Series: Annals of Agricultural and Environmental Medicine
• Subjects: mice; microsporidia; Enterocytozoon bieneusi; Encephalitozoon intestinalis; Encephalitozoon cuniculi; zoonotic potencial
• Online Access: http://www.journalssystem.com/aaem/First-report-of-i-Enterocytozoon-i-bieneusi-and-i-Encephalitozoon-intestinalis-i-infection-of-wild-mice-in-Slovakia,72269,0,2.html
• ISSN: 1232-1966; 1898-2263

Increased risk of zoonotic transmission of the potential human pathogenic species <i>Enterocytozoon bieneusi</i>, <i>Encephalitozoon intestinalis</i> and <i>Encephalitozoon cuniculi</i> was detected in wild immunocompetent mice (Mus musculus musculus; n=280). Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, <i>E. bieneusi</i> was detected in 3 samples (1.07 %), <i>E. cuniculi</i> in 1 sample (0.35 %) and <i>E. intestinalis</i> in 1 sample (0.35 %). The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.