Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva

The value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants...

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Main Authors:	Alexander Rabe, Manuela Gesell Salazar, Stephan Fuchs, Thomas Kocher, Uwe Völker
Format:	Article
Language:	English
Published:	Taylor & Francis Group 2018-01-01
Series:	Journal of Oral Microbiology
Subjects:	Saliva metaproteomics human oral microbiome whole saliva proteomics collection method nLC-MS/MS
Online Access:	http://dx.doi.org/10.1080/20002297.2018.1428006

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spelling	doaj-da32e4222ac24e3e94d57ba657fa7d2d2020-11-25T01:16:30ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972018-01-0110110.1080/20002297.2018.14280061428006Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human salivaAlexander Rabe0Manuela Gesell Salazar1Stephan Fuchs2Thomas Kocher3Uwe Völker4University Medicine GreifswaldUniversity Medicine GreifswaldRobert Koch-InstituteUniversity Medicine GreifswaldUniversity Medicine GreifswaldThe value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants and present a workflow including PG preparation which is suitable for metaproteomics.http://dx.doi.org/10.1080/20002297.2018.1428006Salivametaproteomicshuman oral microbiomewhole saliva proteomicscollection methodnLC-MS/MS
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Alexander Rabe Manuela Gesell Salazar Stephan Fuchs Thomas Kocher Uwe Völker
spellingShingle	Alexander Rabe Manuela Gesell Salazar Stephan Fuchs Thomas Kocher Uwe Völker Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva Journal of Oral Microbiology Saliva metaproteomics human oral microbiome whole saliva proteomics collection method nLC-MS/MS
author_facet	Alexander Rabe Manuela Gesell Salazar Stephan Fuchs Thomas Kocher Uwe Völker
author_sort	Alexander Rabe
title	Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_short	Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_full	Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_fullStr	Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_full_unstemmed	Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
title_sort	comparative analysis of salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva
publisher	Taylor & Francis Group
series	Journal of Oral Microbiology
issn	2000-2297
publishDate	2018-01-01
description	The value of saliva as a diagnostic tool can be increased by taxonomic and functional analyses of the microbiota as recently demonstrated. In this proof-of-principle study, we compare two collection methods (Salivette® (SV) and paraffin gum (PG)) for stimulated saliva from five healthy participants and present a workflow including PG preparation which is suitable for metaproteomics.
topic	Saliva metaproteomics human oral microbiome whole saliva proteomics collection method nLC-MS/MS
url	http://dx.doi.org/10.1080/20002297.2018.1428006
work_keys_str_mv	AT alexanderrabe comparativeanalysisofsalivetteandparaffingumpreparationsforestablishmentofametaproteomicsanalysispipelineforstimulatedhumansaliva AT manuelagesellsalazar comparativeanalysisofsalivetteandparaffingumpreparationsforestablishmentofametaproteomicsanalysispipelineforstimulatedhumansaliva AT stephanfuchs comparativeanalysisofsalivetteandparaffingumpreparationsforestablishmentofametaproteomicsanalysispipelineforstimulatedhumansaliva AT thomaskocher comparativeanalysisofsalivetteandparaffingumpreparationsforestablishmentofametaproteomicsanalysispipelineforstimulatedhumansaliva AT uwevolker comparativeanalysisofsalivetteandparaffingumpreparationsforestablishmentofametaproteomicsanalysispipelineforstimulatedhumansaliva
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Comparative analysis of Salivette® and paraffin gum preparations for establishment of a metaproteomics analysis pipeline for stimulated human saliva

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